FlyBase Allele Report: Dmel\Atg12[HMS01153]

General Information

Symbol

Dmel\Atg12^HMS01153

Species

D. melanogaster

Name

FlyBase ID

FBal0257813

Feature type

allele

Associated gene

Dmel\Atg12

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01153}

Also Known As

Atg12 RNAi, UAS-Atg12^RNAi, UAS-RNAi-Atg12, HMS01153

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01153}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01153

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | oogenesis, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

abnormal cell size | prepupal stage | somatic clone, with Scer\GAL4^Act.PU

(Liu et al., 2016)

abnormal stress response | oogenesis, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

(Zhang et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adulthood-only expression of Atg12^HMS01153 under the control of Scer\GAL4^esg.PU leads to a significant increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

(Zhang et al., 2019)

Adulthood-only expression of Atg12^HMS01153 under the combined control of Scer\GAL4^{Myo31DF-NP0001} and Gal80[ts] does not significantly affect the posterior midgut cell size, as compared to controls. Adulthood-only expression under the combined control of Scer\GAL4^tub.PU and Gal80[ts] leads to the failure to induce autophagy (assessed by the lysosome and autophagosome marker, Lysotracker) in the adult fat body in response to starvation, as compared to controls.

(Wen et al., 2017)

The clonal expression of Atg12^HMS01153 under the control of Scer\GAL4^Act.PU results in a significant increase in the transverse cell area and a significant increase in relative mitochondrial content of midgut enterocytes during pupariation, as compared to controls.

(Liu et al., 2016)

Expression of Atg12^HMS01153 under the control of Scer\GAL4^Mef2.PR is able to significantly suppress the formation of autophagosomes in the muscles of chloroquine-treated larvae starved on low-nutrient food for 6 hours.

(Zirin et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Atg12^HMS01153, Scer\GAL4^GMR.long is a non-suppressor of visible | adult stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Atg12^HMS01153, Scer\GAL4^Act.PU has mitochondrion | prepupal stage | somatic clone phenotype, non-enhanceable | somatic clone by Atg5^KK108904, Scer\GAL4^Act.PU

(Liu et al., 2016)

NOT Suppressor of

Statement

Reference

Atg12^HMS01153, Scer\GAL4^GMR.long is a non-suppressor of eye phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The increased relative mitochondrial content resulting from the clonal expression of Atg12^HMS01153 under the control of Scer\GAL4^Act.PU in midgut enterocytes during pupariation is not significantly enhanced by the clonal co-expression of Atg5^KK108904.

(Liu et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

34675