FlyBase Allele Report: Dmel\drpr[RNAi.UAS]

General Information

Symbol

Dmel\drpr^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0258016

Feature type

allele

Associated gene

Dmel\drpr

Associated Insertion(s)

Carried in Construct

P{UAS-drpr.RNAi}

Also Known As

UAS-draper^RNAi, pUAST-draper-RNAi, UAS-drprRNAi, UAS-drpr^RNAi

Key Links

Transgenic product class

RNAi_reagent

(MacDonald et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(MacDonald et al., 2006)

Mutagen

in vitro construct

(MacDonald et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-drpr.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of a 502bp fragment (nucleotides 1885-2386) of drpr cloned in an inverted repeat in the pWIZ vector.

(MacDonald et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

drpr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.E693G.UAS.VTR}

(Ray et al., 2017)

exacerbates Huntington's disease

modeled by Hsap\HTT^{Q91.ex1.QUAS.mCherry}

(Pearce et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult ensheathing glial cell, with Scer\GAL4^Mz709

(Doherty et al., 2009)

adult ensheathing glial cell, with Scer\GAL4^repo.PU

(Doherty et al., 2009)

adult olfactory receptor neuron, with Scer\GAL4^repo.PU

(MacDonald et al., 2006)

astrocyte-like glial cell, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

axon | adult stage, with Scer\GAL4^repo

(Hsu et al., 2021)

egg chamber | nutrition conditional, with Scer\GAL4^GR1

(Etchegaray et al., 2012)

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

(McPhee et al., 2010)

follicle cell | nutrition conditional, with Scer\GAL4^GR1

(Etchegaray et al., 2012)

gamma Kenyon cell, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

lysosome, with Scer\GAL4^fkh.PH

(McPhee et al., 2010)

wing nerve, with Scer\GAL4^repo

(Hsu et al., 2021)

wing vein L1 | conditional, with Scer\GAL4^Gli-rL82

(Neukomm et al., 2014)

wing vein L1 | conditional, with Scer\GAL4^nrv2.PS

(Neukomm et al., 2014)

wing vein L1 | conditional, with Scer\GAL4^repo

(Neukomm et al., 2014)

Detailed Description

Statement

Reference

Flies expressing drpr^{dsRNA.Scer\UAS} in all glia under the control of Scer\GAL4^repo are unable to clear axonal debris from L1 wing vein neurons following axotomy. Expression in wrapping glia under the control of Scer\GAL4^nrv2.PS also results in strong suppression of clearance of axonal debris, whereas expression in subperineurial glia (under the control of Scer\GAL4^Gli-rL82) has a much more modest effect.

(Neukomm et al., 2014)

Expression of drpr^{dsRNA.Scer\UAS} under the control of Scer\GAL4^alrm.PD results in a strong suppression of mushroom body γ neuron pruning at 18 hours after pupal formation and a mild phenotype in adults.

drpr appears to function in non-astrocyte glia (e.g. ensheathing or cortex glia), since knockdown of drpr, through expression of drpr^{dsRNA.Scer\UAS} under the control of Scer\GAL4^alrm.PD, has no effect on cell body clearance.

(Tasdemir-Yilmaz and Freeman, 2014)

Follicle cell specific expression of drpr^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GR1 prevents the follicle cell enlargement and uptake of nurse cell debris normally seen in starvation-induced degenerating egg chambers.

(Etchegaray et al., 2012)

Expression of drpr^{Scer\UAS.dsRNA} in the hemocytes under the control of Scer\GAL4^Hml.PG does not result in defects in salivary gland clearance.

Expression of drpr^{Scer\UAS.dsRNA} in the salivary glands under the control of Scer\GAL4^fkh.PH results in persistence of salivary gland fragments in 89% of pupae. Salivary glands contain more lysosomes than control salivary glands.

(McPhee et al., 2010)

Expression of drpr^{dsRNA.Scer\UAS} under the control of either Scer\GAL4^repo.PU or Scer\GAL4^Mz709 completely suppresses the recruitment of ensheathing glia membranes to severed olfactory receptor neuron axons after severing of the maxillary palp.

Expression of drpr^{dsRNA.Scer\UAS} under the control of either Scer\GAL4^repo.PU or Scer\GAL4^Mz709 completely blocks glial clearance of axonal debris from the central nervous system after severing of the maxillary palp (axonal debris is efficiently cleared within 5 days after injury in wild-type controls). Expression of drpr^{dsRNA.Scer\UAS} under the control of Scer\GAL4^alrm.PD has no effect on axon debris clearance after severing of the maxillary palp.

(Doherty et al., 2009)

Glial-specific knock-down of drpr through expression of drpr^{Scer\UAS.dsRNA} under the control of Scer\GAL4^repo.PU fully suppresses glial responses to antennal or maxillary palp ablation.

(MacDonald et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^alrm.PD, drpr^RNAi.UAS has abnormal neuroanatomy phenotype, non-enhanceable by Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

NOT Enhancer of

Statement

Reference

drpr^RNAi.UAS, Scer\GAL4^alrm.PD is a non-enhancer of abnormal neuroanatomy phenotype of Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^alrm.PD, drpr^RNAi.UAS has astrocyte-like glial cell phenotype, non-enhanceable by Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Scer\GAL4^alrm.PD, drpr^RNAi.UAS has gamma Kenyon cell phenotype, non-enhanceable by Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

NOT Enhancer of

Statement

Reference

drpr^RNAi.UAS, Scer\GAL4^alrm.PD is a non-enhancer of astrocyte-like glial cell phenotype of Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

drpr^RNAi.UAS, Scer\GAL4^alrm.PD is a non-enhancer of gamma Kenyon cell phenotype of Ced-12^KK102788, Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of drpr^{dsRNA.Scer\UAS} and Ced-12^KK102788 under the control of Scer\GAL4^alrm.PD does not increase the number of cell bodies or the amount of neurite debris when compared with elimination of each pathway alone.

(Tasdemir-Yilmaz and Freeman, 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

67034

y¹ w^*; P{UAS-drpr.RNAi}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Draper^RNAi

(Pearce et al., 2015)

Drpr^RNAi

(Donnelly et al., 2020)

drpr^RNAi.UAS

drpr^{Scer\UAS.dsRNA}

drpr^{dsRNA.Scer\UAS}

drpr^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (22)

Report Sections

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