Expression of Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP in larval body wall sensory neurons under the control of Scer\GAL4RluA1 has no apparent effect on larval da neuron viability.
Expression of Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP in larval body wall sensory neurons under the control of Scer\GAL4RluA1 results in the formation of cytoplasmic aggregates in the axon and cell body, and in addition aggregates are seen throughout the extensive dendritic arbors of the da neurons. There is also some limited aggregation in the nucelus.
Expression of Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP in larval body wall sensory neurons under the control of Scer\GAL4RluA1 does not affect dendrite elaboration in da neurons of the dorsal cluster. Dendrites fully cover the field and project towards the dorsal midline to meet the contralateral dendrites, and also fully extend to segment boundaries.
The speed of locomotion is not different in Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP expressing larva (driven by Scer\GAL4RluA1) compared to wild-type controls. The trajectory of larval locomotion is also not affected.
Expression of Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP in the developing eye using the Scer\GAL4GMR.PF driver does not produce a strong external eye phenotype.
Expression of Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 in the developing eye using the Scer\GAL4GMR.PF driver produces a degenerative eye phenotype characterized by loss of pigment cells and an increased occurrence of dark necrotic spots on the external eye.
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has melanotic necrosis phenotype, suppressible by kriGD11686, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has melanotic necrosis phenotype, suppressible by Nup160GD11413, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has melanotic necrosis phenotype, suppressible by DnaJ-1UAS.cKa, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has melanotic necrosis phenotype, suppressible by Wdr33GD7195, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has eye phenotype, enhanceable by RhebUAS.cPa, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has pigment cell phenotype, enhanceable by RhebUAS.cPa, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has eye phenotype, enhanceable by hiwGD14104, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has pigment cell phenotype, enhanceable by hiwGD14104, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has eye phenotype, suppressible by DnaJ-1UAS.cKa, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has pigment cell phenotype, suppressible by DnaJ-1UAS.cKa, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has eye phenotype, suppressible by Wdr33GD7195, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has pigment cell phenotype, suppressible by Wdr33GD7195, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has eye phenotype, suppressible by kriGD11686, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has pigment cell phenotype, suppressible by kriGD11686, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has eye phenotype, suppressible by Nup160GD11413, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP, Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF has pigment cell phenotype, suppressible by Nup160GD11413, Scer\GAL4GMR.PF
Hsap\HTT152Q.Ex1.UAS.EGFP/Hsap\HTT48Q.Ex1.UAS.EGFP.Tag:NLS(Unk), Scer\GAL4GMR.PF is an enhancer of eye phenotype of Scer\GAL4GMR.PF, hiwGD14101
Expression of DnaJ-1Scer\UAS.cKa in the developing eye under the control of Scer\GAL4GMR.PF suppresses the eye phenotype found in flies mutant for both Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1.
Overexpression of RhebScer\UAS.cPa in the developing eye enhances the phenotype seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF, with a severe rough eye phenotype and enhancement of pigment cell loss.
Expression of CG1109GD7195 in the developing eye under the control of Scer\GAL4GMR.PF strongly suppresses the loss of pigment seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF. There is a clear decrease in the number of necrotic fly eyes in these progeny.
Expression of CG5537GD11686 in the developing eye under the control of Scer\GAL4GMR.PF suppresses the loss of pigment seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF. There is a clear decrease in the number of necrotic fly eyes in these progeny.
Expression of Nup160GD11413 in the developing eye under the control of Scer\GAL4GMR.PF strongly suppresses the loss of pigment seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF. There is a clear decrease in the number of necrotic fly eyes in these progeny.
Expression of hiwGD14104 in the developing eye under the control of Scer\GAL4GMR.PF enhances the loss of pigment seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF.