Both UASt and ms(3)K81 upstream regulatory sequences are fused upstream of a ms(3)K81 open reading frame (amplified from genomic DNA) in which the QFVH motif has been mutated to PTV. The open reading frame is tagged at the N-terminal end with EGFP.
ms(3)K81PTV.UAS.K81.EGFP fails to rescue ms(3)K81unspecified
ms(3)K81PTV.T:Avic\GFP is unable to rescue the sterility of ms(3)K81unspecified mutants.