Amino acid replacement: Y953N.
T8710548A
Y953N | raw-PA; Y769N | raw-PB; Y953N | raw-PC; Y796N | raw-PD; Y953N | raw-PE
Y953N
Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.
abnormal neurophysiology | third instar larval stage | conditional (with rawdcp-1), with Scer\GAL4BG380, rawUASp.RA
axon | third instar larval stage | conditional (with rawdcp-1)
gonad | embryonic stage (with raw155.27)
rawdcp-1/raw134.47 transheterozygotes show protection against (injury-induced) Wallerian degeneration of third instar larval motor neurons.
Unlike controls, rawdcp-1/raw134.47 transheterozygotes expressing rawUASp.RA under the control of Scer\GAL4Toll-6-D42 or Scer\GAL4BG380 sustain some neurotransmission at the third instar larval neuromuscular junction at 20h after injury.
While only 5% of gonads exhibit defects in germ cell ensheathment in heterozygous controls, raw134.47/raw155.27 mutants exhibit ensheathment defects in 45% of gonads. Mutant germ cells appear to form clumps surrounded by somatic gonadal precursor cells at the periphery of the gonad in stage 15 developing embryos.
In control male embryos, approximately 9% of gonads contain at least one mitotic germ cell. In contrast, in raw134.47/raw155.27 mutant male embryos, only 1% of gonads contain mitotic germ cells.
raw134.47/raw155.27 embryos have gonads in which somatic gonadal precursors (SGPs) fail to wrap individual germ cells. Consequently, the unensheathed germ cells are associated in larger clusters rather than being separated by the SGPs. Somatic gonadal precursor specification appears normal. Visceral mesoderm appears normal.
rawdcp-1/raw134.47 is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Hsap\SARM1SAM.TIR.UAS.Venus, Scer\GAL4tey-5053A
raw134.47/raw[+] is a suppressor | partially of increased cell number | oogenesis phenotype of Scer\GAL4C587, Su(var)3-3NIG.17149R
rawdcp-1/raw134.47 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Hsap\SARM1SAM.TIR.UAS.Venus, Scer\GAL4tey-5053A
raw155.27/raw134.47 has germline cell | embryonic stage 15 phenotype, enhanceable by shg[+]/shgg317
raw155.27/raw134.47 has germline cell | embryonic stage 15 phenotype, enhanceable by shgg317
raw155.27/raw134.47 has germline cell | embryonic stage 15 phenotype, suppressible by bskDN.UAS/Scer\GAL4αTub84B.PL
raw155.27/raw134.47 has germline cell | embryonic stage 15 phenotype, suppressible by JraIA109/Jra[+]
raw155.27/raw134.47 has germline cell | embryonic stage 15 phenotype, suppressible by bsk1/bsk[+]
raw155.27/raw134.47 has germline cell | embryonic stage 15 phenotype, suppressible by shgαTub84B.PP
rawdcp-1/raw134.47 is a non-enhancer of axon | larval stage phenotype of Hsap\SARM1SAM.TIR.UAS.Venus, Scer\GAL4tey-5053A
rawdcp-1/raw134.47 is a non-enhancer of embryonic/larval motor neuron | larval stage phenotype of Hsap\SARM1SAM.TIR.UAS.Venus, Scer\GAL4tey-5053A
raw134.47/raw[+] is a suppressor | partially of egg chamber | decreased number phenotype of Scer\GAL4C587, Su(var)3-3NIG.17149R
rawdcp-1/raw134.47 is a non-suppressor of axon | larval stage phenotype of Hsap\SARM1SAM.TIR.UAS.Venus, Scer\GAL4tey-5053A
rawdcp-1/raw134.47 is a non-suppressor of embryonic/larval motor neuron | larval stage phenotype of Hsap\SARM1SAM.TIR.UAS.Venus, Scer\GAL4tey-5053A
Expression of shgαTub84B.PP (presumably in the soma) is sufficient to strongly suppress the ensheathment defects in raw134.47/raw155.27 mutant gonads.
shgg317/+ enhances the frequency of ensheathment defects in raw134.47/raw155.27 mutants. Homozygous shgg317 does so even at a greater frequency.
bsk1/+ suppresses the frequency of ensheathment defects in raw134.47/raw155.27 mutants.
JraIA109/+ suppresses the frequency of ensheathment defects in raw134.47/raw155.27 mutants.
Expression of bskDN.Scer\UAS using Scer\GAL4αTub84B.PL suppresses the frequency of ensheathment defects in raw134.47/raw155.27 mutants.
rawdcp-1/raw134.47 is partially rescued by rawUASp.RA/Scer\GAL4Toll-6-D42
rawdcp-1/raw134.47 is partially rescued by rawUASp.RA/Scer\GAL4tey-5053A
rawdcp-1/raw134.47 is partially rescued by Scer\GAL4tey-5053A/rawUASp.RB
rawdcp-1/raw134.47 is not rescued by rawUASp.RB/Scer\GAL4repo