FlyBase Allele Report: Dmel\nab[EP-nd642]

General Information

Symbol

Dmel\nab^EP-nd642

Species

D. melanogaster

Name

FlyBase ID

FBal0261068

Feature type

allele

Associated gene

Dmel\nab

Associated Insertion(s)

P{EP}nab^EP-nd642

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EP}nab^EP-nd642

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^ap-nd432

(Suissa et al., 2011)

increased cell death, with Scer\GAL4^hh-nd271

(Suissa et al., 2011)

increased cell growth rate | cell non-autonomous | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Suissa et al., 2011)

Phenotype Manifest In

wing disc, with Scer\GAL4^ap-nd432

(Suissa et al., 2011)

wing disc, with Scer\GAL4^hh-nd271

(Suissa et al., 2011)

Detailed Description

Statement

Reference

Over-expression of nab^EP642 in the posterior compartment of the wing imaginal disc under the control of Scer\GAL4^hh.nd271 causes massive cell death. Somatic clones over-expressing nab^EP642 are often eliminated from the wing imaginal disc 48 hours after their induction, indicating that these cells are dying rapidly.

(Suissa et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, nab^EP-nd642 has increased cell growth rate | cell non-autonomous | somatic clone phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Suissa et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^hh-nd271, nab^EP-nd642 has increased cell death phenotype, suppressible by brk^GD1390/Scer\GAL4^hh-nd271

(Suissa et al., 2011)

Scer\GAL4^hh-nd271, nab^EP-nd642 has increased cell death phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^hh-nd271

(Suissa et al., 2011)

Scer\GAL4^ap-nd432, nab^EP-nd642 has increased cell death phenotype, suppressible by Df(3L)H99

(Suissa et al., 2011)

Scer\GAL4^ap-nd432, nab^EP-nd642 has increased cell death phenotype, suppressible by Df(3L)XR38

(Suissa et al., 2011)

Scer\GAL4^ap-nd432, nab^EP-nd642 has increased cell death phenotype, suppressible by hid⁰⁵⁰¹⁴

(Suissa et al., 2011)

Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, nab^EP-nd642 has increased cell growth rate | somatic clone | cell non-autonomous phenotype, suppressible by puc^UAS.cMa

(Suissa et al., 2011)

Scer\GAL4^ap-nd432, nab^EP-nd642 has increased cell death phenotype, suppressible by Diap1^EP.M, Scer\GAL4^ap-nd432

(Suissa et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ap-nd432, nab^EP-nd642 has increased cell death phenotype, non-suppressible by grim^GD11287

(Suissa et al., 2011)

Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, nab^EP-nd642 has increased cell growth rate | somatic clone | cell non-autonomous phenotype, non-suppressible by Df(3L)H99

(Suissa et al., 2011)

Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, nab^EP-nd642 has increased cell growth rate | somatic clone | cell non-autonomous phenotype, non-suppressible by Dronc^NIG.8091R, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Suissa et al., 2011)

Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, nab^EP-nd642 has increased cell growth rate | somatic clone | cell non-autonomous phenotype, non-suppressible by Diap1^EP.M, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Suissa et al., 2011)

Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, nab^EP-nd642 has increased cell death | somatic clone | cell non-autonomous phenotype, non-suppressible by p53^D259H.UAS/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Suissa et al., 2011)

Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, nab^EP-nd642 has increased cell growth rate | somatic clone | cell non-autonomous phenotype, non-suppressible by Df(3L)H99/p53^D259H.UAS, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Suissa et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, nab^EP-nd642 has wing disc | cell non-autonomous | somatic clone phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Suissa et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^ap-nd432, nab^EP-nd642 has wing disc phenotype, suppressible by Df(3L)XR38

(Suissa et al., 2011)

Scer\GAL4^ap-nd432, nab^EP-nd642 has wing disc phenotype, suppressible by hid⁰⁵⁰¹⁴

(Suissa et al., 2011)

Scer\GAL4^{FRT.Rnor\Cd2.Act5C}, nab^EP-nd642 has wing disc | somatic clone | cell non-autonomous phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Suissa et al., 2011)

Scer\GAL4^hh-nd271, nab^EP-nd642 has wing disc phenotype, suppressible by brk^GD1390/Scer\GAL4^hh-nd271

(Suissa et al., 2011)

Scer\GAL4^hh-nd271, nab^EP-nd642 has wing disc phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^hh-nd271

(Suissa et al., 2011)

Scer\GAL4^ap-nd432, nab^EP-nd642 has wing disc phenotype, suppressible by Df(3L)H99

(Suissa et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ap-nd432, nab^EP-nd642 has wing disc phenotype, non-suppressible by grim^GD11287

(Suissa et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The massive cell death seen upon over-expression of nab^EP642 in the posterior compartment of the wing imaginal disc under the control of Scer\GAL4^hh.nd271 is suppressed by knock down of brk through concurrent expression of brk^GD1390.

Ectopic expression of puc^Scer\UAS.cMa blocks nab^EP642-induced apoptosis in the imaginal wing disc.

nab^EP642-induced apoptosis in the dorsal compartment of the wing imaginal disc (using the Scer\GAL4^ap.nd432 driver) is virtually abolished upon removal of one copy of rpr, W and grim through a Df(3L)H99 background.

Removal of one copy of rpr through a Df(3L)XR38 heterozygous background significantly attenuates nab^EP642-induced apoptosis in the dorsal compartment of the wing imaginal disc (using the Scer\GAL4^ap.nd432 driver).

Removal of one copy of W through a W⁰⁵⁰¹⁴ heterozygous background significantly attenuates nab^EP642-induced apoptosis in the dorsal compartment of the wing imaginal disc (using the Scer\GAL4^ap.nd432 driver).

No change in nab^EP642-induced apoptosis in the dorsal compartment of the wing imaginal disc (using the Scer\GAL4^ap.nd432 driver) is found upon co-expression of grim^GD11287.

Clones of cells co-expressing nab^EP642 and puc^Scer\UAS.cMa in the wing imaginal disc (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) exhibit a block on overgrowth.

Clones of cells expressing nab^EP642 in the wing imaginal disc (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) in a Df(3L)H99 heterozygous background stimulate a strong non-autonomous growth of neighboring tissue, as evidenced by wing disc overgrowth.

Clones of homozygous Df(3L)H99 wing disc cells over-expressing nab^EP642 (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) exhibit compensatory proliferation, as evidenced by wing disc overgrowth.

Clones of cells expressing nab^EP642 in the wing imaginal disc (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) co-expressing Nc^NIG.8091-R1 display strong JNK activity associated with robust extra tissue growth.

Co-expression of Nc^NIG.8091-R1 in clones of cells expressing nab^EP642 in the wing imaginal disc (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) efficiently suppresses effector caspase activity but compensatory proliferation of neighboring cells still occurs.

Co-expression of th^EP.M and nab^EP642 in the wing imaginal disc under the control of Scer\GAL4^ap.nd432 abolishes effector caspase activation and apoptosis.

Co-expression of th^EP.M in clones of cells expressing nab^EP642 in the wing imaginal disc (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) fails to suppress nab^EP642-induced tissue overgrowth.

Apoptotic cell elimination induced by nab^EP642 expression in wing imaginal disc clones (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) is not affected by co-expression of p53^{D259H.Scer\UAS}.

Clones of imaginal wing disc cells co-expressing both nab^EP642 and p53^{D259H.Scer\UAS} (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) in a Df(3L)H99 heterozygous background exhibit non-autonomous tissue overgrowth.

(Suissa et al., 2011)

Xenogenetic Interactions

Statement

Reference

Clones of cells expressing nab^EP642 and BacA\p35^Scer\UAS.cHa in the wing imaginal disc (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) exhibit a strong non-autonomous proliferation stimulatory effect, as evidenced by wing imaginal disc overgrowth.

(Suissa et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nab^EP-nd642

nab^EP642

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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