FlyBase Allele Report: Dmel\Ncc69[UAS.Tag:HA]

General Information

Symbol

Dmel\Ncc69^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0261185

Feature type

allele

Associated gene

Dmel\Ncc69

Associated Insertion(s)

Carried in Construct

P{UAS-HA-Ncc69}

Also Known As

UAS-Ncc69

Key Links

Transgenic product class

wild_type

(Leiserson et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Leiserson et al., 2011)

Mutagen

in vitro construct

(Leiserson et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-HA-Ncc69}

Cytology

Description

UASt regulatory sequences drive expression of Ncc69 which is tagged with a Tag:HA epitope.

(Leiserson et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ncc69

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates episodic ataxia type 6

modeled by Hsap\SLC1A3^PR.UAS.Venus

(Parinejad et al., 2016)

modeled by Eaat1^PR.UAS.Venus

(Parinejad et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cold stress response | larval stage, with Scer\GAL4^19-12

(Himmel et al., 2023)

abnormal locomotor behavior | first instar larval stage, with Scer\GAL4^repo.PU

(Parinejad et al., 2016)

abnormal neuroanatomy | first instar larval stage, with Scer\GAL4^repo.PU

(Parinejad et al., 2016)

abnormal neurophysiology | larval stage, with Scer\GAL4^19-12

(Himmel et al., 2023)

Phenotype Manifest In

astrocyte-like glial cell | first instar larval stage, with Scer\GAL4^repo.PU

(Parinejad et al., 2016)

larval multidendritic class III neuron, with Scer\GAL4^19-12

(Himmel et al., 2023)

neuropil | first instar larval stage, with Scer\GAL4^repo.PU

(Parinejad et al., 2016)

Detailed Description

Statement

Reference

Expression of Ncc69^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^repo.PU in L1 larvae leads to increased astrocyte infiltration of the neuropil and increased locomotor performance, as compared to controls.

(Parinejad et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ncc69^UAS.Tag:HA, Scer\GAL4^repo.PU is a suppressor of abnormal locomotor behavior | first instar larval stage phenotype of Eaat1^PR.UAS.Venus, Scer\GAL4^repo.PU

(Parinejad et al., 2016)

Ncc69^UAS.Tag:HA, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | first instar larval stage phenotype of Eaat1^PR.UAS.Venus, Scer\GAL4^repo.PU

(Parinejad et al., 2016)

Ncc69^UAS.Tag:HA/Scer\GAL4^Gli-rL82.29 is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Ncc69^r2

(Leiserson et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Ncc69^UAS.Tag:HA, Scer\GAL4^repo.PU is a suppressor of astrocyte-like glial cell | first instar larval stage phenotype of Eaat1^PR.UAS.Venus, Scer\GAL4^repo.PU

(Parinejad et al., 2016)

Ncc69^UAS.Tag:HA, Scer\GAL4^repo.PU is a suppressor of neuropil | first instar larval stage phenotype of Eaat1^PR.UAS.Venus, Scer\GAL4^repo.PU

(Parinejad et al., 2016)

Ncc69^UAS.Tag:HA/Scer\GAL4^Gli-rL82.29 is a suppressor | partially of abdominal nerve | third instar larval stage phenotype of Ncc69^r2

(Leiserson et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ncc69^{Scer\UAS.T:Ivir\HA1} suppresses the reduced astrocyte infiltration and paralysis caused by expression of Eaat1^{PR.Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^repo.PU. Astrocyte numbers in these larvae are unchanged as compared to controls.

(Parinejad et al., 2016)

Xenogenetic Interactions

Statement

Reference

Co-expression of Ncc69^{Scer\UAS.T:Ivir\HA1} partially suppresses the reduced astrocyte infiltration and paralysis caused by expression of Hsap\SLC1A3^{PR.Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^repo.PU.

(Parinejad et al., 2016)

Complementation and Rescue Data

Rescues

Ncc69^UAS.Tag:HA/Scer\GAL4^Gli-rL82.29 rescues Ncc69^r2

(Leiserson et al., 2011)

Partially rescues

Ncc69^UAS.Tag:HA/Scer\GAL4^P2.4.Pdf partially rescues Ncc69^r2

(Schellinger et al., 2022)

Fails to rescue

Ncc69^UAS.Tag:HA/Scer\GAL4^Gli-rL82.29 fails to rescue Ncc69^r2

(Schellinger et al., 2022)

Comments

Expression of Ncc69^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Gli-rL82.29 rescues the bulges seen in the abdominal nerves of Ncc69^r2 larvae.

(Leiserson et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ncc69^{Scer\UAS.T:Ivir\HA1}

Ncc69^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (6)

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