FlyBase Allele Report: Dmel\cnc[RNAi.C.UAS]

General Information

Symbol

Dmel\cnc^RNAi.C.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0263742

Feature type

allele

Associated gene

Dmel\cnc

Associated Insertion(s)

Carried in Construct

P{UAS-cnc.C.RNAi}

Also Known As

UAS-cncC-RNAi, UAS cncC^RNAi, UAS-cncC^RNAi, UAS cncC RNAi, UAS-CncC RNAi

Key Links

Transgenic product class

RNAi_reagent

(Sykiotis and Bohmann, 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Sykiotis and Bohmann, 2008)

Mutagen

in vitro construct

(Sykiotis and Bohmann, 2008)

Progenitor genotype

Carried in construct

P{UAS-cnc.C.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat targeting the longest isoform (C) of cnc for RNAi mediated knockdown.

(Sykiotis and Bohmann, 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

cnc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult abdomen, with Scer\GAL4^ct.CtB

(Burbridge et al., 2021)

adult Malpighian tubule, with Scer\GAL4^ct.CtB

(Burbridge et al., 2021)

embryonic/larval Malpighian tubule | larval stage, with Scer\GAL4^ct.CtB

(Burbridge et al., 2021)

Detailed Description

Statement

Reference

The size, morphology and number of nuclei in the prothoracic gland is normal in larvae expressing cnc^{dsRNA.C.Scer\UAS} under the control of Scer\GAL4⁵⁰¹⁵.

Expression of cnc^{dsRNA.C.Scer\UAS} under the control of either Scer\GAL4^phm.PO or Scer\GAL4⁵⁰¹⁵ results in delayed pupation compared to controls.

Feeding with 20-hydroxyecdysone rescues the delayed pupation seen in larvae expressing cnc^{dsRNA.C.Scer\UAS} under the control of Scer\GAL4^phm.PO.

Pupae expressing cnc^{dsRNA.C.Scer\UAS} under the control of Scer\GAL4^phm.PO are larger than control larvae.

(Deng and Kerppola, 2013)

Expression of cnc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Switch.tub significantly decreases the survival of both sexes after exposure to oxidative stress.

(Sykiotis and Bohmann, 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ct.CtB, cnc^RNAi.C.UAS has short lived phenotype, suppressible | partially by Cat^UAS.cAa, Scer\GAL4^ct.CtB

(Burbridge et al., 2021)

Scer\GAL4^phtm.PO, cnc^RNAi.C.UAS has increased body size | pupal stage phenotype, suppressible by Ras85D^V12.UAS, Scer\GAL4^phtm.PO

(Deng and Kerppola, 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^ct.CtB, cnc^RNAi.C.UAS has short lived phenotype, non-suppressible by Sod2^UAS.cMa/Scer\GAL4^ct.CtB

(Burbridge et al., 2021)

Suppressor of

Statement

Reference

cnc^RNAi.C.UAS, Scer\GAL4⁵⁰¹⁵ is a suppressor | partially of lethal phenotype of Ras85D^V12.UAS, Scer\GAL4⁵⁰¹⁵

(Deng and Kerppola, 2013)

cnc^RNAi.C.UAS, Scer\GAL4^phtm.PO is a suppressor of decreased body size | pupal stage phenotype of Ras85D^V12.UAS, Scer\GAL4^phtm.PO

(Deng and Kerppola, 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ct.CtB, cnc^RNAi.C.UAS has adult Malpighian tubule phenotype, enhanceable by Sod2^UAS.cMa/Scer\GAL4^ct.CtB

(Burbridge et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^ct.CtB, cnc^RNAi.C.UAS has adult Malpighian tubule phenotype, suppressible | partially by Cat^UAS.cAa, Scer\GAL4^ct.CtB

(Burbridge et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of cnc^{dsRNA.C.Scer\UAS} in animals expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4⁵⁰¹⁵ allows the majority of animals to develop to late pupal stages, and some eclose as adults.

Co-expression of cnc^{dsRNA.C.Scer\UAS} suppresses the small body size of pupae expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^phm.PO.

(Deng and Kerppola, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

cnc^RNAi.C.UAS

cnc^{dsRNA.C.Scer\UAS}

cnc^dsRNA.C.UAS

cnc^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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