FlyBase Allele Report: Dmel\Oamb[unspecified]

General Information

Symbol

Dmel\Oamb^unspecified

Species

D. melanogaster

Name

FlyBase ID

FBal0263793

Feature type

allele

Associated gene

Dmel\Oamb

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | male

(Zhou et al., 2012)

abnormal learning | male

(Zhou et al., 2012)

female semi-sterile | recessive

(Lee et al., 2009)

Phenotype Manifest In

oviduct

(Lee et al., 2009)

uterus

(Lee et al., 2009)

Detailed Description

Statement

Reference

Mutant males show significantly impaired courtship conditioning compared to wild-type males.

(Zhou et al., 2012)

The percentage of flies that have an egg present in the oviduct or uterus following mating is reduced in Oamb^unspecified 5-7 day old homozygous flies compared to controls.

(Lee et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Oamb^unspecified has female semi-sterile | recessive phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^Oamb.4.4

(Lee et al., 2009)

NOT suppressed by

Statement

Reference

Oamb^unspecified has female semi-sterile | recessive phenotype, non-suppressible by Scer\GAL4^elav.PU/CaMKII^T287D.UAS/Scer\GAL80^ts.αTub84B

(Lee et al., 2009)

Oamb^unspecified has female semi-sterile | recessive phenotype, non-suppressible by Mmus\Prkaca^mC.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^Oamb.4.4

(Lee et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Oamb^unspecified has oviduct phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^Oamb.4.4

(Lee et al., 2009)

Oamb^unspecified has uterus phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^Oamb.4.4

(Lee et al., 2009)

NOT suppressed by

Statement

Reference

Oamb^unspecified has oviduct phenotype, non-suppressible by Scer\GAL4^elav.PU/CaMKII^T287D.UAS/Scer\GAL80^ts.αTub84B

(Lee et al., 2009)

Oamb^unspecified has uterus phenotype, non-suppressible by Scer\GAL4^elav.PU/CaMKII^T287D.UAS/Scer\GAL80^ts.αTub84B

(Lee et al., 2009)

Oamb^unspecified has oviduct phenotype, non-suppressible by Mmus\Prkaca^mC.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^Oamb.4.4

(Lee et al., 2009)

Oamb^unspecified has uterus phenotype, non-suppressible by Mmus\Prkaca^mC.UAS/Scer\GAL80^ts.αTub84B/Scer\GAL4^Oamb.4.4

(Lee et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^Oamb.4.4 in a Oamb^unspecified heterozygous background show wild type ovulation levels. Wild type ovulation levels are also seen when Scer\GAL80^ts.αTub84B is used to limit expression to 3 days prior to mating.

Flies expressing inaC^{PKCi.Scer\UAS} under the control of Scer\GAL4^Oamb.4.4 in a Oamb^unspecified heterozygous background show wild type ovulation levels. Wild type ovulation levels are also seen when Scer\GAL80^ts.αTub84B is used to limit expression to 3 days prior to mating.

Expression of CaMKII^{T287D.Scer\UAS} under the control of Scer\GAL4^Oamb.4.4, using Scer\GAL80^ts.αTub84B to limit expression to 3 days prior to mating, suppresses the ovulation phenotype seen in Oamb^unspecified homozygotes.

Expression of CaMKII^{T287D.Scer\UAS} under the control of Scer\GAL4^elav.PU, using Scer\GAL80^ts.αTub84B to limit expression to 3 days prior to mating, fails to suppress the ovulation phenotype seen in Oamb^unspecified homozygotes.

(Lee et al., 2009)

Xenogenetic Interactions

Statement

Reference

Flies expressing Rat\CamKII-I^Ala.hs under the control of Scer\GAL4^Oamb.4.4 in a Oamb^unspecified heterozygous background show significantly reduced ovulation levels compared to controls. Reduced ovulation levels are also seen when Scer\GAL80^ts.αTub84B is used to limit expression to 3 days prior to mating.

Expression of Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^Oamb.4.4, using Scer\GAL80^ts.αTub84B to limit expression to 3 days prior to mating, fails to suppress the ovulation phenotype seen in Oamb^unspecified homozygotes.

(Lee et al., 2009)

Complementation and Rescue Data

Rescued by

Oamb^unspecified is rescued by Scer\GAL4^elav.PU/Oamb^UAS.cZa

(Zhou et al., 2012)

Oamb^unspecified is rescued by Oamb^UAS.cZa/Scer\GAL4^c739

(Zhou et al., 2012)

Oamb^unspecified is rescued by Scer\GAL4^hs.PB/Oamb^UAS.K3

(Lee et al., 2009)

Oamb^unspecified is rescued by Scer\GAL4^Oamb.4.4/Oamb^UAS.K3

(Lee et al., 2009)

Oamb^unspecified is rescued by Oamb^UAS.AS/Scer\GAL4^Oamb.4.4/Oamb^UAS.K3

(Lee et al., 2009)

Oamb^unspecified is rescued by Scer\GAL80^ts.αTub84B/Scer\GAL4^Oamb.4.4/Oamb^UAS.K3

(Lee et al., 2009)

Oamb^unspecified is rescued by Oamb^UAS.AS/Scer\GAL4^hs.PB

(Lee et al., 2009)

Oamb^unspecified is rescued by Oamb^UAS.AS/Scer\GAL4^Oamb.4.4

(Lee et al., 2009)

Oamb^unspecified is rescued by Oamb^UAS.AS/Scer\GAL80^ts.αTub84B/Scer\GAL4^Oamb.4.4

(Lee et al., 2009)

Partially rescued by

Oamb^unspecified is partially rescued by Oamb^UAS.AS/Oamb^UAS.K3

(Lee et al., 2009)

Oamb^unspecified is partially rescued by Oamb^UAS.AS/Scer\GAL4^elav.PU/Oamb^UAS.K3

(Lee et al., 2009)

Not rescued by

Oamb^unspecified is not rescued by Scer\GAL4^elav.PU/Oamb^UAS.K3

(Lee et al., 2009)

Oamb^unspecified is not rescued by Scer\GAL4^ChAT.7.4/Oamb^UAS.K3

(Lee et al., 2009)

Oamb^unspecified is not rescued by Oamb^UAS.AS/Scer\GAL4^elav.PU

(Lee et al., 2009)

Oamb^unspecified is not rescued by Oamb^UAS.AS/Scer\GAL4^ChAT.7.4

(Lee et al., 2009)

Comments

The courtship conditioning defects seen in Oamb^unspecified males are rescued by expression of Oamb^Scer\UAS.cZa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^c739.

(Zhou et al., 2012)

Expression of Oamb^Scer\UAS.K3 under the control of the Scer\GAL4^hs.PB driver rescues the ovulation defects seen in Oamb^unspecified mutants following heat shock at 37[o]C for 1h twice a day for 3 days. No rescue is seen when either construct is expressed alone or if no heat shock is applied.

Expression of Oamb^Scer\UAS.K3 in neurons using the Scer\GAL4^elav.PU driver fails to rescue the ovulation defects seen in Oamb^unspecified mutants.

Co-expression of Oamb^Scer\UAS.AS and Oamb^Scer\UAS.K3 in the absence of a Scer\GAL4 driver partially rescues the ovulation defects seen in Oamb^unspecified mutants. The phenotype is not suppressed any further through the addition of the Scer\GAL4^elav.PU neuronal driver.

Expression of Oamb^Scer\UAS.K3 in neurons using the Scer\GAL4^Cha.7.4 driver fails to rescue the ovulation defects seen in Oamb^unspecified mutants.

Expression of Oamb^Scer\UAS.K3 in the oviduct epithelium using the Scer\GAL4^Oamb.4.4 driver rescues the ovulation defects seen in Oamb^unspecified mutants.

Co-expression of Oamb^Scer\UAS.AS and Oamb^Scer\UAS.K3 in the oviduct epithelium using Scer\GAL4^Oamb.4.4 rescues the ovulation defects seen in Oamb^unspecified mutants.

Expression of Oamb^Scer\UAS.K3 under the control of Scer\GAL4^Oamb.4.4, using Scer\GAL80^ts.αTub84B to limit expression to 3 days prior to mating, results in rescue of the ovulation defects seen in Oamb^unspecified mutants.

Expression of Oamb^Scer\UAS.AS under the control of the Scer\GAL4^hs.PB driver rescues the ovulation defects seen in Oamb^unspecified mutants following heat shock at 37[o]C for 1h twice a day for 3 days. No rescue is seen when either construct is expressed alone, however partial rescue is seen when Oamb^Scer\UAS.AS and Scer\GAL4^hs.PB are both present but no heat shock is applied.

Expression of Oamb^Scer\UAS.AS in neurons using the Scer\GAL4^elav.PU driver fails to rescue the ovulation defects seen in Oamb^unspecified mutants.

Expression of Oamb^Scer\UAS.AS in neurons using the Scer\GAL4^Cha.7.4 driver fails to rescue the ovulation defects seen in Oamb^unspecified mutants.

Expression of Oamb^Scer\UAS.AS in the oviduct epithelium using the Scer\GAL4^Oamb.4.4 driver rescues the ovulation defects seen in Oamb^unspecified mutants.

Expression of Oamb^Scer\UAS.AS under the control of Scer\GAL4^Oamb.4.4, using Scer\GAL80^ts.αTub84B to limit expression to 3 days prior to mating, results in rescue of the ovulation defects seen in Oamb^unspecified mutants.

(Lee et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Oamb^unspecified

Name Synonyms

Secondary FlyBase IDs

References (2)

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