FB2024_03 , released June 25, 2024
Allele: Dmel\OambUAS.K3
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General Information
Symbol
Dmel\OambUAS.K3
Species
D. melanogaster
Name
FlyBase ID
FBal0263791
Feature type
allele
Associated gene
Dmel\Oamb
Associated Insertion(s)
Carried in Construct
P{UAS-Oamb.K3}
Also Known As
UAS-K3
Key Links
Transgenic product class
cDNA
(Lee et al., 2009)
wild_type
(Lee et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Lee et al., 2009)
wild_type
(Lee et al., 2009)
Mutagen
in vitro construct
(Lee et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Oamb.K3}
Cytology
Description

UAS regulatory sequences drive expression of Oamb-RB cDNA.

(Lee et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Oamb
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Females overexpressing OambUAS.K3 under the control of Scer\GAL4GMR47A04 shows a significant increase in egg chamber follicle rupture (ex vivo, octopamine-treated egg chambers), as compared to controls.

      (Deady et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4Oamb.4.4/OambUAS.K3 is a non-suppressor of female sterile | recessive phenotype of Octβ2Rf05679

      (Lim et al., 2014)
      Phenotype Manifest In
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Ilp2.PR/OambUAS.K3 rescues Oamb286

      (Crocker et al., 2010)

      Scer\GAL4hs.PB/OambUAS.K3 rescues Oambunspecified

      (Lee et al., 2009)

      Scer\GAL4Oamb.4.4/OambUAS.K3 rescues Oambunspecified

      (Lee et al., 2009)

      OambUAS.AS, Scer\GAL4Oamb.4.4, OambUAS.K3 rescues Oambunspecified

      (Lee et al., 2009)

      Scer\GAL80ts.αTub84B, Scer\GAL4Oamb.4.4, OambUAS.K3 rescues Oambunspecified

      (Lee et al., 2009)
      Partially rescues

      OambUAS.AS/OambUAS.K3 partially rescues Oambunspecified

      (Lee et al., 2009)

      OambUAS.AS, Scer\GAL4elav.PU, OambUAS.K3 partially rescues Oambunspecified

      (Lee et al., 2009)
      Fails to rescue

      Scer\GAL4elav.PU/OambUAS.K3 fails to rescue Oambunspecified

      (Lee et al., 2009)

      Scer\GAL4ChAT.7.4/OambUAS.K3 fails to rescue Oambunspecified

      (Lee et al., 2009)
      Comments

      Expression of OambScer\UAS.K3 in Scer\GAL4Ilp2.PR neurons results in a significant reduction in sleep in a Oamb286 background, thus effectively rescuing the phenotype. A decrease in nighttime sleep in response to 10 mg/ml octopamine is also rescued in these flies.

      (Crocker et al., 2010)

      Expression of OambScer\UAS.K3 under the control of the Scer\GAL4hs.PB driver rescues the ovulation defects seen in Oambunspecified mutants following heat shock at 37[o]C for 1h twice a day for 3 days. No rescue is seen when either construct is expressed alone or if no heat shock is applied.

      Expression of OambScer\UAS.K3 in neurons using the Scer\GAL4elav.PU driver fails to rescue the ovulation defects seen in Oambunspecified mutants.

      Co-expression of OambScer\UAS.AS and OambScer\UAS.K3 in the absence of a Scer\GAL4 driver partially rescues the ovulation defects seen in Oambunspecified mutants. The phenotype is not suppressed any further through the addition of the Scer\GAL4elav.PU neuronal driver.

      Expression of OambScer\UAS.K3 in neurons using the Scer\GAL4Cha.7.4 driver fails to rescue the ovulation defects seen in Oambunspecified mutants.

      Expression of OambScer\UAS.K3 in the oviduct epithelium using the Scer\GAL4Oamb.4.4 driver rescues the ovulation defects seen in Oambunspecified mutants.

      Co-expression of OambScer\UAS.AS and OambScer\UAS.K3 in the oviduct epithelium using Scer\GAL4Oamb.4.4 rescues the ovulation defects seen in Oambunspecified mutants.

      Expression of OambScer\UAS.K3 under the control of Scer\GAL4Oamb.4.4, using Scer\GAL80ts.αTub84B to limit expression to 3 days prior to mating, results in rescue of the ovulation defects seen in Oambunspecified mutants.

      (Lee et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      OambScer\UAS.K3
      OambUAS.K3
      Name Synonyms
      Secondary FlyBase IDs
        References (5)