UAS regulatory sequences drive expression of the two JIL-1 kinase domains (amino acids 255-888) tagged with Tag:V5 at the C-terminal end and with a nuclear localization signal (Tag:NLS(SV40-largeT)) at the N-terminal end.
JIL-1KDI-KDII.UAS.Tag:V5,Tag:NLS(SV40-largeT)/Scer\GAL4hs.PB partially rescues JIL-1z2
Expression of JIL-1KDI-KDII.Scer\UAS.T:SV5\V5,T:SV40\nls2 under the control of the Scer\GAL4hs.PB driver partially rescues the uncoiling and loss of banding phenotypes seen in the autosomal polytene chromosomes of JIL-1z2 homozygous larvae. The phenotypes seen in the male X chromosome are not rescued. The Scer\GAL4hs.PB driver is leaky and therefore no heat shock is required.