UAS regulatory sequences drive expression of amino acids 927-1207 of JIL-1 tagged with ECFP and a nuclear localization signal (Tag:NLS(SV40-largeT)).
Expression of JIL-1CTD.Scer\UAS.T:Avic\GFP-ECFP,T:SV40\nls2 under the control of the Scer\GAL4hs.PB driver rescues both the autosomal and male X polytene chromosome phenotypes seen in JIL-1z2 homozygous larvae. The Scer\GAL4hs.PB driver is leaky and therefore no heat shock is required.