Two amino acid replacements (S88A and S91A) have been introduced into grhUAS.cKa.
Expression of grh2A.Scer\UAS under the control of Scer\GAL4Act5C.PU, Scer\GAL4da.G32 or Scer\GAL4arm.PS results in lethality at the embryonic or larval stages.
Flies expressing grh2A.Scer\UAS in the epidermis under the control of Scer\GAL4e22c survive to adulthood.
grh2A.UAS/Scer\GAL4e22c partially rescues grhIM
Expression of grh2A.Scer\UAS in the epidermis under the control of Scer\GAL4e22c partially rescues the cuticle and head skeleton defects seen in grhIM embryos.
Expression of grh2A.Scer\UAS in the epidermis under the control of Scer\GAL4e22c rescues the defects in epidermal barrier integrity seen in stage 17 grhIM homozygous embryos. Epidermal barrier regeneration following wounding is only partially rescued.