FlyBase Allele Report: Dmel\vret[148-60]

General Information

Symbol

Dmel\vret^148-60

Species

D. melanogaster

Name

FlyBase ID

FBal0265156

Feature type

allele

Associated gene

Dmel\vret

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Zamparini et al., 2011)

Mutagen

ethyl methanesulfonate

(Zamparini et al., 2011)

Progenitor genotype

Cytology

Description

Amino acid replacement: I536L.

(Zamparini et al., 2011)

Amino acid replacement: Q537K.

(Zamparini et al., 2011)

Amino acid replacement: L636term.

(Zamparini et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:22,743,372..22,743,372 [-]

Nucleotide change:

T22743372A

Amino acid change:

L636term | vret-PA

Reported amino acid change:

L636term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zamparini et al., 2011)

point_mutation

3R:22,743,729..22,743,729 [-]

Nucleotide change:

A22743729C

Amino acid change:

I536L | vret-PA

Reported amino acid change:

I536L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zamparini et al., 2011)

point_mutation

3R:22,743,726..22,743,726 [-]

Nucleotide change:

C22743726A

Amino acid change:

Q537K | vret-PA

Reported amino acid change:

Q537K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zamparini et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Zamparini et al., 2011)

increased cell death

(Zamparini et al., 2011)

male sterile

(Zamparini et al., 2011)

Phenotype Manifest In

dorsal appendage | germline clone

(Zamparini et al., 2011)

escort cell

(Zamparini et al., 2011)

spectrosome

(Zamparini et al., 2011)

Detailed Description

Statement

Reference

vret^148-60 females lay no eggs. The germarium is filled with undifferentiated germ cells that maintain a round spectrosome and inner sheath cells fail to associate with germ cells.

Generation of vret^148-60 germline clones results in a ventralized eggshell phenotype (fused/missing dorsal appendage), but does not affect earlier aspects of germ cell or somatic differentiation. Generation of vret^148-60 somatic follicle cell clones has no effect on oocyte specification or localization or the morphology of the follicle cell epithelium.

Inner sheath cells and accompanying somatic cells, which normally intermingle with germ cells in wild type, die in vret^148-60 mutant ovaries.

(Zamparini et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference