FlyBase Allele Report: Dmel\Trim9[91]

General Information

Symbol

Dmel\Trim9⁹¹

Species

D. melanogaster

Name

FlyBase ID

FBal0266852

Feature type

allele

Associated gene

Dmel\Trim9

Associated Insertion(s)

Carried in Construct

Also Known As

asap⁹¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Morikawa et al., 2011)

Progenitor genotype

P{GawB}Trim9^NP4638

(Morikawa et al., 2011)

Cytology

Description

Imprecise excision of the P{GawB} element in P{GawB}Trim9^NP4638 resulted in a 1.2kb deletion 8bp downstream of the insertion site. A three base insertion (CAT) remains in the deleted region.

(Morikawa et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:10,545,260..10,546,460 [+]

Comment:

1.2 kb deletion resulting from the imprecise excision of P{GawB}Trim9^NP4638, starts 8bp from insertion site and extends into Trim9 gene. Downstream breakpoint approximate.

(Morikawa et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage

(Morikawa et al., 2011)

abnormal neuroanatomy | embryonic stage

(Morikawa et al., 2011)

abnormal neuroanatomy | embryonic stage (with Df(2L)Exel8026)

(Morikawa et al., 2011)

abnormal neuroanatomy | larval stage

(Morikawa et al., 2011)

abnormal neuroanatomy | larval stage (with Df(2L)Exel8026)

(Morikawa et al., 2011)

Phenotype Manifest In

larval dorsal multidendritic neuron ddaC | third instar larval stage (with Df(2L)Exel8026)

(Morikawa et al., 2011)

larval multidendritic class IV neuron | embryonic stage

(Morikawa et al., 2011)

larval multidendritic class IV neuron | embryonic stage (with Df(2L)Exel8026)

(Morikawa et al., 2011)

larval multidendritic class IV neuron | third instar larval stage

(Morikawa et al., 2011)

larval multidendritic class IV neuron | third instar larval stage (with Df(2L)Exel8026)

(Morikawa et al., 2011)

larval ventral multidendritic neuron vdaB | third instar larval stage (with Df(2L)Exel8026)

(Morikawa et al., 2011)

larval ventral nerve cord commissure | embryonic stage

(Morikawa et al., 2011)

larval ventral nerve cord commissure | embryonic stage (with Df(2L)Exel8026)

(Morikawa et al., 2011)

larval ventral nerve cord commissure | third instar larval stage

(Morikawa et al., 2011)

larval ventral nerve cord commissure | third instar larval stage (with Df(2L)Exel8026)

(Morikawa et al., 2011)

Detailed Description

Statement

Reference

Homozygous Trim9⁹¹ third instar larvae show a marked loss of C4da commissural fascicles in the ventral nerve cord. Axonal defects are visible by embryonic stage 17.

Trim9⁹¹/Df(2L)Exel8026 third instar larvae show a marked loss of C4da commissural fascicles in the ventral nerve cord. Axonal defects are visible by embryonic stage 17.

In contrast to wild type flies, almost no contralateral projections of the ddaC and vdaB axons are seen in Trim9⁹¹/Df(2L)Exel8026 third instar larvae. However, the frequency of longitudinal branches is comparable to that in wild type.

3D image reconstruction shows that, as in wild type, the commissural branches in all subtypes of Trim9⁹¹/Df(2L)Exel8026 C4da neurons are composed of highly branched and tangled processes. However, the wild type terminal processes are oriented almost exclusively to the medial side in all subtypes, whereas in Trim9⁹¹/Df(2L)Exel8026 mutants the processes are oriented in both medial and lateral directions.

Visualisation of presynaptic terminals using Syt1^{Scer\UAS.T:Avic\GFP-EGFP} shows that Trim9⁹¹ ddaC neurons (generated using the MARCM system under the control of Scer\GAL4^ppk.PG) only form synaptic connections on the ipsilateral side of the commissural branches, compared to both the ipsilateral and contralateral sides in wild type.

Homozygous Trim9⁹¹ third instar larvae crawl with significantly fewer head turning behaviors compared to wild type. In addition, a concomitant increase in crawling speed is observed in Trim9⁹¹ mutants.

(Morikawa et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Trim9⁹¹ is partially rescued by Trim9^UAS.cMa/Scer\GAL4^ppk.PG

(Morikawa et al., 2011)

Trim9⁹¹/Df(2L)Exel8026 is partially rescued by Trim9^UAS.cMa/Scer\GAL4^ppk.PG

(Morikawa et al., 2011)

Trim9⁹¹/Df(2L)Exel8026 is partially rescued by Trim9^UAS.ΔRING/Scer\GAL4^ppk.PG

(Morikawa et al., 2011)

Trim9⁹¹/Df(2L)Exel8026 is partially rescued by Scer\GAL4^ppk.PG/Trim9^UAS.ΔBB

(Morikawa et al., 2011)

Not rescued by

Trim9⁹¹/Df(2L)Exel8026 is not rescued by Trim9^UAS.ΔCC/Scer\GAL4^ppk.PG

(Morikawa et al., 2011)

Trim9⁹¹/Df(2L)Exel8026 is not rescued by Trim9^UAS.ΔFNIII/Scer\GAL4^ppk.PG

(Morikawa et al., 2011)

Trim9⁹¹/Df(2L)Exel8026 is not rescued by Trim9^UAS.ΔSPRY/Scer\GAL4^ppk.PG

(Morikawa et al., 2011)

Comments

C4da neuron-specific expression of Trim9^Scer\UAS.cMa under the control of Scer\GAL4^ppk.PG largely rescues the axonal defects seen in Trim9⁹¹/Df(2L)Exel8026 mutants.

Expression of Trim9^{Scer\UAS.ΔFNIII} under the control of Scer\GAL4^ppk.PG is unable to rescue the axonal projection defects seen in Trim9⁹¹/Df(2L)Exel8026 C4da neurons.

Expression of Trim9^{Scer\UAS.ΔSPRY} under the control of Scer\GAL4^ppk.PG is unable to rescue the axonal projection defects seen in Trim9⁹¹/Df(2L)Exel8026 C4da neurons.

Expression of Trim9^{Scer\UAS.ΔCC} under the control of Scer\GAL4^ppk.PG is unable to rescue the axonal projection defects seen in Trim9⁹¹/Df(2L)Exel8026 C4da neurons.

Expression of Trim9^{Scer\UAS.ΔBB} under the control of Scer\GAL4^ppk.PG partially rescues the axonal projection defects seen in Trim9⁹¹/Df(2L)Exel8026 C4da neurons.

Expression of Trim9^{Scer\UAS.ΔRING} under the control of Scer\GAL4^ppk.PG partially rescues the axonal projection defects seen in Trim9⁹¹/Df(2L)Exel8026 C4da neurons.

Expression of Trim9^Scer\UAS.cMa under the control of Scer\GAL4^ppk.PG significantly rescues the crawling behavior defects seen in Trim9⁹¹ mutants.

(Morikawa et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Trim9⁹¹

asap⁹¹

(Li et al., 2019, Morikawa et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close