FB2024_03 , released June 25, 2024
Allele: Dmel\Toll-6ex13
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General Information
Symbol
Dmel\Toll-6ex13
Species
D. melanogaster
Name
FlyBase ID
FBal0267031
Feature type
allele
Associated gene
Dmel\Toll-6
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Yagi et al., 2010)
Progenitor genotype
Toll-6GS11682
(Yagi et al., 2010)
Cytology
Description

Imprecise excision of P{GSV6}Toll-6GS11682 results in a deletion of the Toll-6 coding region, from approximately 450bp upstream of the transcription start site to between 40 and 50kb downstream of the coding region.

(Yagi et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      VA1d olfactory receptor neuron axons mistarget either dorsomedially or dorsolaterally in 50% of cases in mutant animals.

      The VA1d and DC3 projection neuron dendrites of adNb clones mistarget extensively to dorsomedial and ventral regions in the antennal lobe. Mistargeting of dendrites is also seen in single-cell VA1d or DC3 projection neuron clones.

      Homozygous DA1 projection neurons in lateral neuroblast clones show local medial mistargeting at a low penetrance.

      Homozygous VA1v projection neurons in ventral neuroblast clones mistarget dendrites to the medial antennal lobe (100% penetrance).

      Homozygous single cell DM6 projection neuron clones target their dendrites normally.

      Expression of Toll-6Scer\UAS.cYa under the control of Scer\GAL4Mz19 in lateral neuroblast clones in homozygous Toll-6ex13 animals results in extensive intermingling of VA1d olfactory receptor neuron axons and DA1 projection neuron dendrites.

      (Ward et al., 2015)

      Septic injury of Toll-6ex13 mutant flies results in activation of the innate immune response.

      (Yagi et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Toll-6ex13 has abnormal neuroanatomy | adult stage phenotype, suppressible by Toll-7UAS.cYa/Scer\GAL4Mz19

      (Ward et al., 2015)
      Enhancer of
      Other
      Statement
      Reference

      18wk02701/18wΔ7-35, Toll-6ex13 has lethal phenotype

      (Yagi et al., 2010)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Toll-7Scer\UAS.cYa under the control of Scer\GAL4Mz19 rescues the VA1d olfactory receptor neuron axon targeting defects seen in Toll-6ex13 mutants.

      Expression of Toll-7Scer\UAS.cYa under the control of Scer\GAL4Mz19 also rescues the mistargeting of VA1d and DC3 projection neuron dendrites which is seen in Toll-6ex13 adNb clones.

      Expression of Toll-7Scer\UAS.cYa under the control of Scer\GAL4AM29 in homozygous Df(2R)Toll-7AW1 animals results in extensive intermingling of mistargeted DM6 olfactory receptor neuron axons with Scer\GAL4Mz19-positive projection neuron dendrites. This intermingling is still seen if the flies are also carrying Toll-6ex13 mutant adNb clones.

      (Ward et al., 2015)

      18wΔ7-35/18wk02701; Toll-6ex13/Toll-6ex13 double mutants display 66% lethality.

      (Yagi et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Toll-6ex13 is rescued by Toll-6Δcyto.UAS.GFP/Scer\GAL4Mz19

      (Ward et al., 2015)

      Toll-6ex13 is rescued by Toll-6TIR-dead.UAS.Tag:FLAG,Tag:HA,Tag:polyHis/Scer\GAL4Mz19

      (Ward et al., 2015)

      Toll-6ex13 is rescued by Toll-6UAS.cYa/Scer\GAL4Mz19

      (Ward et al., 2015)
      Comments

      Expression of Toll-6Scer\UAS.cYa under the control of Scer\GAL4Mz19 rescues the dorsal mistargeting of VA1d axons that is seen in Toll-6ex13 mutants. Rescue of the phenotype is still seen if expression of Scer\GAL4Mz19 is limited to the VA1d and DC3 projection neurons using MARCM clonal analysis.

      Expression of Toll-6Scer\UAS.cYa under the control of Scer\GAL4Mz19 also rescues the mistargeting of VA1d and DC3 projection neuron dendrites which is seen in Toll-6ex13 adNb clones.

      Expression of either Toll-6Δcyto.Scer\UAS.T:Avic\GFP or Toll-6TIR-dead.Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1,T:Zzzz\His6 under the control of Scer\GAL4Mz19 rescues the mistargeting phenotypes seen in the Scer\GAL4Mz19-positive projection neuron dendrites and VA1d olfactory receptor axons of Toll-6ex13 animals.

      (Ward et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Toll-6ex13
      (Jetti et al., 2023, Kamemura et al., 2022, Casas-Tintó et al., 2017, Yagi et al., 2010)
      toll-6Ex13
      (Ward et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)