The expression of myoUAS.cAa under the control of either Scer\GAL4Mef2.PR or Scer\GAL4repo results in a significant decrease in the average larval weight, as compared to controls; however, there is no defect in developmental time to pupariation upon Scer\GAL4Mef2.PR-driven expression. The expression under the control of Scer\GAL4Mef2.PR, but not of Scer\GAL4repo, also results in a significant decrease in the size of larval muscles 6 and 7, as compared to controls. The expression under the control of Scer\GAL4repo, but not of Scer\GAL4Mef2.PR, also results in a significantly increased larval crawling capacity, as compared to controls.
The neurotransmission across the larval muscle 6 neuromuscular junction (NMJ) is impaired upon the expression of myoUAS.cAa under the control of Scer\GAL4Mef2.PR, as show by the significant decrease in the amplitude of excitatory junctional currents (eEJCs) despite of no changes in the miniature junctional currents (mEJC) frequency and amplitude, as compared to controls; this expression also leads to a small but significant decrease in the NMJ length, but not in the number of branches or in the density of presynaptic active zones (Brp-positive puncta), as compared to controls. Expression under the control of Scer\GAL4repo also leads to a decrease in neurotransmission across the larval NMJ, as show by the small but significant decreases in the eEJC amplitude and in the mEJC cumulative amplitude, as compared to controls. Neurotransmission across the adult TTM neuron NMJ, but not the DLM neuron NMJ, is also affected by the expression of myoUAS.cAa under the control of Scer\GAL4sli.PH, exhibiting a significant increase in response latency upon electric stimulation of giant fibers, but not upon electric stimulation of the abdomen, as compared to controls.
When myoScer\UAS.cAa is expressed under the control of both Scer\GAL4myo.PA and Scer\GAL80repo.PL in homozygous myoΔ1 animals, mushroom bodies of the mutant pharate adults fail to undergo normal remodeling.
Scer\GAL4repo.PU, myoUAS.cAa has lethal phenotype, suppressible | partially by Scer\GAL4repo.PU/plumΔCyt.UAS.Tag:FLAG
Scer\GAL4repo.PU/myoUAS.cAa is a suppressor | partially of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4repo.PU, plumΔCyt.UAS.Tag:FLAG
Scer\GAL4repo.PU/myoUAS.cAa is a suppressor | partially of gamma Kenyon cell phenotype of Scer\GAL4repo.PU, plumΔCyt.UAS.Tag:FLAG
The lethality caused by expression of myoScer\UAS.cAa under the control of Scer\GAL4repo.PU is partially suppressed by co-expression of plumΔCyt.Scer\UAS.T:Zzzz\FLAG : 31% of animals survive to adulthood. Co-expression of myoScer\UAS.cAa partially suppresses the axon pruning defect see in the mushroom body γ neurons of animals expressing plumΔCyt.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4repo.PU.
Scer\GAL4myo.PA/myoUAS.cAa partially rescues myoΔ1
When myoScer\UAS.cAa is expressed under the control of Scer\GAL4myo.PA in homozygous myoΔ1 animals, the mutants grow into pharate or eclosed adults. The mushroom body undergoes normal remodeling in the rescued animals.