FB2024_03 , released June 25, 2024
Allele: Dmel\PGRP-LFUAS.GFP
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General Information
Symbol
Dmel\PGRP-LFUAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0267522
Feature type
allele
Associated gene
Dmel\PGRP-LF
Associated Insertion(s)
Carried in Construct
P{UAS-PGRP-LF.GFP}
Key Links
Transgenic product class
wild_type
(Maillet et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Maillet et al., 2008)
Mutagen
in vitro construct
(Maillet et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-PGRP-LF.GFP}
Cytology
Description

UAS regulatory sequences drive expression of PGRP-LF tagged with GFP.

(Maillet et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
PGRP-LF
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of PGRP-LFScer\UAS.T:Avic\GFP under the control of Scer\GAL4hs.PB or Scer\GAL4yolk blocks IMD pathway activation after infection and renders flies insensitive to infection by a number of Gram-Negative bacteria such as E. cloacae or E. carotovara. No effects were seen on Toll pathway activation by Gram-positive bacteria.

      Expression of PGRP-LFScer\UAS.T:Avic\GFP under the control of Scer\GAL4hs.PB or Scer\GAL4yolk blocks IMD pathway activation after infection with E. coli. Expression of PGRP-LFScer\UAS.T:Avic\GFP under the control of Scer\GAL4yolk blocks IMD pathway activation after injection with highly purified PGN from E.coli.

      IMD pathway activation is not blocked when PGRP-LFScer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4hs.PB after injection with Lys-type PGN from S.aureus.

      (Maillet et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4ap-md544/PGRP-LFUAS.GFP rescues PGRP-LF200

      (Maillet et al., 2008)
      Partially rescues

      PGRP-LFUAS.GFP/Scer\GAL4hs.PB partially rescues PGRP-LFRNAi.1.UAS

      (Maillet et al., 2008)

      PGRP-LFUAS.GFP/Scer\GAL4hs.PB partially rescues PGRP-LFRNAi.2.UAS

      (Maillet et al., 2008)
      Comments

      Expression of PGRP-LFScer\UAS.T:Avic\GFP partially rescues the infection-independent immune response seen when PGRP-LFdsRNA.1.Scer\UAS is expressed under the control of Scer\GAL4hs.PB.

      Expression of PGRP-LFScer\UAS.T:Avic\GFP partially rescues the infection-independent immune response seen when PGRP-LFdsRNA.2.Scer\UAS is expressed under the control of Scer\GAL4hs.PB.

      Expression of PGRP-LFScer\UAS.T:Avic\GFP under the control of Scer\GAL4ap-md544 rescues the wing notching defects seen in PGRP-LF200 mutant flies.

      (Maillet et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PGRP-LFScer\UAS.T:Avic\GFP
      PGRP-LFUAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)