UASt regulatory sequences drive expression of Nrx-IV which lacks sequence encompassing laminin G domain 2, the following EGF repeats and laminin G domain 3 and sequence encompassing laminin G domain 4, the following EGF repeats and laminin G domain 5. The Nrx-IV coding sequence is tagged with 6 copies of Tag:MYC after nucleotide 3590.
Scer\GAL4arm.PS/Nrx-IVΔLEL1-LEL2.UAS.Tag:MYC fails to rescue Nrx-IV4304
Scer\GAL4Act5C.PU/Nrx-IVΔLEL1-LEL2.UAS.Tag:MYC fails to rescue Nrx-IV4304
Expression of Nrx-IVΔLEL1-LEL2.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4arm.PS fails to rescue the formation of septate junctions in Nrx-IV4304/Nrx-IV4304 embryos.
The transepithelial barrier defect seen in the salivary glands of Nrx-IV4304/Nrx-IV4304 embryos is not rescued by expression of Nrx-IVΔLEL1-LEL2.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PU.