FlyBase Allele Report: Hsap\TARDBP[UAS.Tag:HA]

General Information

Symbol

Hsap\TARDBP^UAS.Tag:HA

Species

H. sapiens

Name

FlyBase ID

FBal0267715

Feature type

allele

Associated gene

Hsap\TARDBP

Associated Insertion(s)

Carried in Construct

P{UAS-TDP-43.HA}

Also Known As

TDP-43

Key Links

Transgenic product class

wild_type

(Gregory et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Gregory et al., 2012)

Mutagen

in vitro construct

(Gregory et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-TDP-43.HA}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\TARDBP tagged with an N-terminal Tag:HA from a construct that allows site-specific integration into the genome.

(Gregory et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\TARDBP

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Gregory et al., 2012)

model of amyotrophic lateral sclerosis type 10

CEA

(Gregory et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of neurodegenerative disease

is ameliorated by CG14207^EP1348

(Gregory et al., 2012)

model of amyotrophic lateral sclerosis type 10

is ameliorated by Hsap\CLU^UAS.cGa

(Gregory et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

17-AAG treatment suppresses neurodegeneration in rough eye model of neurodegenerative disease.

(Gregory et al., 2012)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^Toll-6-D42, Scer\GAL80^ts.αTub84B

(Gregory et al., 2012)

short lived, with Scer\GAL4^Toll-6-D42, Scer\GAL80^ts.αTub84B

(Gregory et al., 2012)

visible, with Scer\GAL4^GMR.long

(Gregory et al., 2012)

visible | adult stage, with Scer\GAL4^GMR.PF

(Crippa et al., 2016)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.long

(Gregory et al., 2012)

eye, with Scer\GAL4^GMR.PF

(Crippa et al., 2016)

ommatidium, with Scer\GAL4^GMR.long

(Gregory et al., 2012)

Detailed Description

Statement

Reference

Expression of Hsap\TARDBP^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF leads to moderate rough eye phenotype.

(Crippa et al., 2016)

Adulthood-only expression of Hsap\TARDBP^{Scer\UAS.T:Ivir\HA1} under the combined control of Scer\GAL4^Toll-6-D42, Scer\GAL80^ts.αTub84B leads to decreased locomotor activity and a decrease in lifespan compared to controls.

(Gregory et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Hsap\TARDBP^UAS.Tag:HA, Scer\GAL4^GMR.PF has visible | adult stage phenotype, non-enhanceable by Hsp67Bc^UAS.Tag:V5, Scer\GAL4^GMR.PF

(Crippa et al., 2016)

Suppressed by

Statement

Reference

Hsap\TARDBP^UAS.Tag:HA, Scer\GAL4^Toll-6-D42, Scer\GAL80^ts.αTub84B has short lived phenotype, suppressible | partially by CG14207^EP1348, Scer\GAL4^Toll-6-D42, Scer\GAL80^ts.αTub84B

(Gregory et al., 2012)

Hsap\TARDBP^UAS.Tag:HA, Scer\GAL4^Toll-6-D42, Scer\GAL80^ts.αTub84B has abnormal locomotor behavior phenotype, suppressible | partially by CG14207^EP1348, Scer\GAL4^Toll-6-D42, Scer\GAL80^ts.αTub84B

(Gregory et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Hsap\TARDBP^UAS.Tag:HA, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Hsp67Bc^UAS.Tag:V5, Scer\GAL4^GMR.PF

(Crippa et al., 2016)

Suppressed by

Statement

Reference

Hsap\TARDBP^UAS.Tag:HA, Scer\GAL4^GMR.long has ommatidium phenotype, suppressible | partially by CG14207^EP1348, Scer\GAL4^GMR.long

(Gregory et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The moderate rough eye phenotype characteristic for adult flies expressing Hsap\TARDBP^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF is not significantly modified by co-expression of Hsp67Bc^{Scer\UAS.T:SV5\V5}.

(Crippa et al., 2016)

Overexpression of CG14207^EP1348 under the control of Scer\GAL4^GMR.long partially suppresses the ommatidium phenotype caused by Hsap\TARDBP^{Scer\UAS.T:Ivir\HA1} expression.

Overexpression of CG14207^EP1348 at the permissive temperature under the control of both Scer\GAL4^D42 and Scer\GAL80^ts.αTub84B partially suppresses the shortened lifespan phenotype caused by Hsap\TARDBP^{Scer\UAS.T:Ivir\HA1} expression alone.

Overexpression of CG14207^EP1348 at the permissive temperature under the control of both Scer\GAL4^D42 and Scer\GAL80^ts.αTub84B partially suppresses the locomotor defects caused by Hsap\TARDBP^{Scer\UAS.T:Ivir\HA1} expression alone.

(Gregory et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\TARDBP^{Scer\UAS.T:Ivir\HA1}

Hsap\TARDBP^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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