UASt regulatory sequences drive expression of an N-terminally truncated 25 kDa fragment (TDP-25) of Hsap\TARDBP tagged with an N-terminal Tag:HA from a construct that allows site-specific integration into the genome. The encoded polypeptide is also lacking the caspase-cleavage site between residues 216-219 of Hsap\TARDBP.
17-AAG treatment suppresses neurodegeneration in rough eye model of neurodegenerative disease.
Adulthood-only expression of Hsap\TARDBPTDP-25.Scer\UAS.T:Ivir\HA1 under the combined control of Scer\GAL4Toll-6-D42, Scer\GAL80ts.αTub84B leads to decreased locomotor activity and a decrease in lifespan compared to controls.
Hsap\TARDBPTDP-25.UAS.Tag:HA, Scer\GAL4Toll-6-D42, Scer\GAL80ts.αTub84B has short lived phenotype, suppressible by CG14207EP1348, Scer\GAL4Toll-6-D42, Scer\GAL80ts.αTub84B
Hsap\TARDBPTDP-25.UAS.Tag:HA, Scer\GAL4Toll-6-D42, Scer\GAL80ts.αTub84B has abnormal locomotor behavior phenotype, suppressible by CG14207EP1348, Scer\GAL4Toll-6-D42, Scer\GAL80ts.αTub84B
Hsap\TARDBPTDP-25.UAS.Tag:HA, Scer\GAL4GMR.long has ommatidium phenotype, suppressible by CG14207EP1348, Scer\GAL4GMR.long
Hsap\TARDBPTDP-25.UAS.Tag:HA, Scer\GAL4GMR.long has ommatidium phenotype, suppressible by CG14207EP1348/Scer\GAL4GMR.PF
Overexpression of CG14207EP1348 under the control of Scer\GAL4GMR.long completely suppresses the ommatidium phenotype caused by Hsap\TARDBPTDP-25.Scer\UAS.T:Ivir\HA1 expression alone.
Overexpression of CG14207EP1348 under the control of Scer\GAL4GMR.PF completely suppresses the ommatidium phenotype caused by Hsap\TARDBPTDP-25.Scer\UAS.T:Ivir\HA1 expression alone.
Overexpression of CG14207EP1348 at the permissive temperature under the control of both Scer\GAL4D42 and Scer\GAL80ts.αTub84B completely suppresses the shortened lifespan phenotype caused by Hsap\TARDBPTDP-25.Scer\UAS.T:Ivir\HA1 expression alone.
Overexpression of CG14207EP1348 at the permissive temperature under the control of both Scer\GAL4D42 and Scer\GAL80ts.αTub84B completely suppresses the locomotor defects caused by Hsap\TARDBPTDP-25.Scer\UAS.T:Ivir\HA1 expression alone.