FlyBase Allele Report: Dmel\RpS6[RNAi.Sym.UAS]

General Information

Symbol

Dmel\RpS6^RNAi.Sym.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0268400

Feature type

allele

Associated gene

Dmel\RpS6

Associated Insertion(s)

Carried in Construct

P{UAS-RpS6.RNAi.Sym}

Key Links

Transgenic product class

RNAi_reagent

(Lin et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Lin et al., 2011)

Mutagen

in vitro construct

(Lin et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-RpS6.RNAi.Sym}

Cytology

Description

UAS regulatory sequences positioned at either end of the coding region drive expression of a 654bp RpS6 fragment in both sense and antisense orientation, resulting in the generation of dsRNA.

(Lin et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

RpS6

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^amn-c651

(Lin et al., 2011)

increased body size | heat sensitive, with Scer\GAL4^amn-c651, Scer\GAL80^ts.αTub84B

(Lin et al., 2011)

visible, with Scer\GAL4^GMR.PU

(Lin et al., 2011)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Lin et al., 2011)

prothoracic gland, with Scer\GAL4^amn-c651

(Lin et al., 2011)

Detailed Description

Statement

Reference

Expression of RpS6^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^GMR.PU results in a smaller eye than normal which has a glassy appearance and necrotic patches.

Expression of RpS6^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^ey.PU does not result in an obvious mutant eye phenotype.

Expression of RpS6^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^amn-c651 results in a reduction in the size of the prothoracic gland in larvae compared with controls at the equivalent time point of 5 days after egg deposition (AED). In addition, these animals show an extreme developmental delay compared to wild type: at day 5 AED the larvae are smaller than control larvae, and they continue to grow and feed beyond day 10 to become giant larvae that fail to pupate. Addition of 20-hydroxyecdysone to the food results in a variable restoration of pupariation, but the final steps of metamorphosis, including the formation of adult structures, are not rescued.

Animals in which expression of RpS6^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^amn-c651 is delayed until the late second larval instar (using the temperature sensitive Scer\GAL80^ts.αTub84B allele) are able to undergo pupation and eclose as large adults. These animals show an increase in eye size and in wing size compared to controls. If the animals are raised in the presence of 20-hydroxyecdysone, the body size of the adults is restored to normal.

(Lin et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

RpS6^RNAi.Sym.UAS/Scer\GAL4^ey.PU is an enhancer of visible phenotype of CycE^JP

(Lin et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/RpS6^RNAi.Sym.UAS is a suppressor of visible phenotype of CycE^JP

(Lin et al., 2011)

RpS6^RNAi.Sym.UAS, Scer\GAL80^ts.αTub84B, Scer\GAL4^amn-c651 is a suppressor of visible phenotype of CycE^JP

(Lin et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

RpS6^RNAi.Sym.UAS/Scer\GAL4^ey.PU is an enhancer of eye phenotype of CycE^JP

(Lin et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/RpS6^RNAi.Sym.UAS is a suppressor of eye phenotype of CycE^JP

(Lin et al., 2011)

RpS6^RNAi.Sym.UAS, Scer\GAL80^ts.αTub84B, Scer\GAL4^amn-c651 is a suppressor of eye phenotype of CycE^JP

(Lin et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of RpS6^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^GMR.PU in a CycE^JP homozygous background results in an additive reduction of eye size.

Expression of RpS6^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^ey.PU enhances the CycE^JP rough eye phenotype.

Expression of RpS6^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^Act.PU suppresses the CycE^JP eye phenotype.

Expression of RpS6^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^amn-c651 from the late second larval instar (expression restricted at earlier stages using the temperature sensitive Scer\GAL80^ts.αTub84B allele) partially suppresses the CycE^JP small eye phenotype.

(Lin et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

RpS6^RNAi.Sym.UAS

RpS6^{dsRNA.Sym.Scer\UAS}

RpS6^{dsRNA.Sym.UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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