FB2024_03 , released June 25, 2024
Allele: Dmel\GgaRNAi.UAS.1
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General Information
Symbol
Dmel\GgaRNAi.UAS.1
Species
D. melanogaster
Name
FlyBase ID
FBal0269003
Feature type
allele
Associated gene
Dmel\Gga
Associated Insertion(s)
Carried in Construct
P{UAS-Gga.RNAi.1}
Key Links
Transgenic product class
RNAi_reagent
(Eissenberg et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Eissenberg et al., 2011)
Mutagen
in vitro construct
(Eissenberg et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-Gga.RNAi.1}
Cytology
Description

UAS regulatory sequences drive expression of an RNAi construct targeting Gga at nucleotides 980-1865 of the cDNA.

(Eissenberg et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Gga
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  neurodegenerative disease
      CEA
      (Eissenberg et al., 2011)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When GgadsRNA.Scer\UAS.1 is expressed under the control of Scer\GAL4Act5C.PU, fewer flies than expected survive to adulthood.

      Expression of GgadsRNA.Scer\UAS.1 under the control of Scer\GAL4GMR.PF results in a degenerative retinal phenotype in aging flies. Photoreceptor organisation is disrupted in 25-day-old flies expressing GgadsRNA.Scer\UAS.1. In addition, these flies show large vacuoles and lacunae in the lamina cortex.

      The external morphology (ommatidial arrays, apical bristle number and organization) of the eye of flies expressing GgadsRNA.Scer\UAS.1 under the control of both Scer\GAL4ey.PH and Scer\GAL4GMR.PF is indistinguishable of that of wild-type flies. However, progressive degeneration of photoreceptor cells is observed in aging mutants including an apparent loss of rhabdomere and pigment granules.

      (Eissenberg et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Suppressor of
      Statement
      Reference

      GgaRNAi.UAS.1, Scer\GAL4GMR.PF is a suppressor of visible phenotype of GgaUAS.GFP, Scer\GAL4GMR.PF

      (Eissenberg et al., 2011)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      GgaRNAi.UAS.1, Scer\GAL4GMR.PF is an enhancer of eye phenotype of Scer\GAL4GMR.PF, bchsEP2299

      (Eissenberg et al., 2011)
      Suppressor of
      Statement
      Reference

      GgaRNAi.UAS.1, Scer\GAL4GMR.PF is a suppressor of eye phenotype of GgaUAS.GFP, Scer\GAL4GMR.PF

      (Eissenberg et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The bchsEP2299-overexpression phenotype in the eye is enhanced when GgadsRNA.Scer\UAS.1 is co-expressed under the control of Scer\GAL4GMR.PF resulting in a reduction in eye size and a marked bleaching in the middle of the eye.

      Co-expression of GgadsRNA.Scer\UAS.1 under the control of Scer\GAL4GMR.PF ameliorates the rough eye phenotype caused by GgaScer\UAS.T:Avic\GFP-overexpression.

      (Eissenberg et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      GgaRNAi.UAS.1
      GgadsRNA.Scer\UAS.1
      GgadsRNA.UAS.1
      Name Synonyms
      Secondary FlyBase IDs
        References (2)