FB2024_03 , released June 25, 2024
Allele: Dmel\GgaRNAi.UAS.2
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General Information
Symbol
Dmel\GgaRNAi.UAS.2
Species
D. melanogaster
Name
FlyBase ID
FBal0269004
Feature type
allele
Associated gene
Dmel\Gga
Associated Insertion(s)
Carried in Construct
P{UAS-Gga.RNAi.2}
Key Links
Transgenic product class
RNAi_reagent
(Eissenberg et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Eissenberg et al., 2011)
Mutagen
in vitro construct
(Eissenberg et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-Gga.RNAi.2}
Cytology
Description

UAS regulatory sequences drive expression of an RNAi construct targeting Gga at nucleotides 287-883 of the cDNA.

(Eissenberg et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Gga
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  lysosomal storage disease
      CEA
      (Eissenberg et al., 2011)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of GgadsRNA.Scer\UAS.2 under the control of Scer\GAL4GMR.PU results in age-dependent retinal degeneration.

      No adult flies expressing P{UAS-Gga.RNAi.2} under the control of Scer\GAL4Act5C.PU in a GgaΔ background are recovered. This illustrates that the lethality associated with expression of P{UAS-Gga.RNAi.2} under the control of Scer\GAL4Act5C.PU must be an off-target effect (not due to an effect on Gga), since the Gga sequences targeted by the P{UAS-Gga.RNAi.2} construct are completely deleted in the GgaΔ allele.

      (Luan et al., 2012)

      When GgadsRNA.Scer\UAS.2 is expressed under the control of any one of Scer\GAL4Act5C.PU, Scer\GAL4mat.αTub67C.T:Hsim\VP16 or Scer\GAL4da.G32, no adults emerge. The lethality occurs during early pupation with all three drivers.

      (Eissenberg et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      GgaRNAi.UAS.2, Scer\GAL4GMR.PF is a suppressor of visible phenotype of GgaUAS.GFP, Scer\GAL4GMR.PF

      (Eissenberg et al., 2011)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      GgaRNAi.UAS.2, Scer\GAL4GMR.PF is a suppressor of eye phenotype of GgaUAS.GFP, Scer\GAL4GMR.PF

      (Eissenberg et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of GgadsRNA.Scer\UAS.2 under the control of Scer\GAL4GMR.PF ameliorates the rough eye phenotype caused by GgaScer\UAS.T:Avic\GFP-overexpression.

      (Eissenberg et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      GgaRNAi.UAS.2
      GgadsRNA.Scer\UAS.2
      GgadsRNA.UAS.2
      Name Synonyms
      Secondary FlyBase IDs
        References (3)