FlyBase Allele Report: Dmel\Ack[UAS.cSa]

General Information

Symbol

Dmel\Ack^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Sem

FlyBase ID

FBal0269477

Feature type

allele

Associated gene

Dmel\Ack

Associated Insertion(s)

Carried in Construct

P{UAS-Ack.S}

Also Known As

UAS-DACK

Key Links

Transgenic product class

cDNA

(Sem et al., 2002)

wild_type

(Sem et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sem et al., 2002)

wild_type

(Sem et al., 2002)

Mutagen

in vitro construct

(Sem et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Ack.S}

Cytology

Description

UASt regulatory sequences drive expression of a wild-type Ack cDNA.

(Sem et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ack

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

embryo | dorsal closure stage, with Scer\GAL4^ptc-559.1

(Sem et al., 2002)

embryo | dorsal closure stage | heat sensitive, with Scer\GAL4^hs.PB

(Sem et al., 2002)

embryonic/first instar larval cuticle | heat sensitive, with Scer\GAL4^hs.PB

(Sem et al., 2002)

eye, with Scer\GAL4^GMR.PF

(Sem et al., 2002)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Sem et al., 2002)

ommatidium, with Scer\GAL4^GMR.PF

(Sem et al., 2002)

Detailed Description

Statement

Reference

A low frequency of mild defects in dorsal closure is seen in embryos expressing Ack^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1.

Expression of Ack^Scer\UAS.cSa under the control of Scer\GAL4^hs.PB in 6 to 12 hour embryos using a 1 hour heat shock results in approximately half the embryos failing to survive to the first larval instar stage. Of the dead embryos, 45% have germband retraction failures, 20% have holes in the dorsal cuticle (indicating failure of dorsal closure) and 25% do not secrete any cuticle.

Flies expressing Ack^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF have rough eyes which are smaller than normal. The ommatidia are disorganised and consistently missing in a band running dorsoventrally across the middle of the eye. The pattern of interommatidial bristles is disrupted, with bristles being missing in some areas of the eye and excessive in numbers in others.

(Sem et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Ack^UAS.cSa/Scer\GAL4^hs.PB is a suppressor of embryo | dorsal closure stage phenotype of tkv⁷

(Zahedi et al., 2008)

Ack^UAS.cSa/Scer\GAL4^c381 is a suppressor of embryo | dorsal closure stage phenotype of tkv⁷

(Zahedi et al., 2008)

Ack^UAS.cSa, Scer\GAL4^hs.PB is a suppressor | heat sensitive | partially of embryonic/first instar larval cuticle | heat sensitive phenotype of Cdc42^N17.UAS, Scer\GAL4^hs.PB

(Sem et al., 2002)

Ack^UAS.cSa, Scer\GAL4^hs.PB is a suppressor | heat sensitive | partially of embryo | dorsal closure stage | heat sensitive phenotype of Cdc42^N17.UAS, Scer\GAL4^hs.PB

(Sem et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ack^Scer\UAS.cSa partially suppresses the dorsal closure defects caused by expression of Cdc42^N17.Scer\UAS under the control of Scer\GAL4^hs.PB using heat shock.

(Sem et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ack^Scer\UAS.cSa

Ack^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sem

Secondary FlyBase IDs

References (4)

Report Sections

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