FlyBase Allele Report: Scer\GAL4[Ddc.HL9]

General Information

Symbol

Scer\GAL4^Ddc.HL9

Species

S. cerevisiae

Name

FlyBase ID

FBal0270073

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Ddc-GAL4.HL9}

Also Known As

HL9-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Claridge-Chang et al., 2009)

Progenitor genotype

Carried in construct

P{Ddc-GAL4.HL9}

Cytology

Description

A genomic fragment from -2702 to +970bp relative to the Ddc transcription start site is fused upstream of the GAL4 coding region. An Hsp70 termination/polyA region and the Ddc exon D 3' untranslated region and 3' flanking sequences (nucleotides +3521 to +4643) are present downstream of the GAL4 coding region. The AUG codon present in the 5' Ddc fragment has been mutated to prevent interference with the translation of GAL4.

(Claridge-Chang et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

Ddc

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

ScerGAL4^Ddc.HL9 drives expression in a subset of the dopaminergic PPL2ab neurons and in the majority of the PAM neurons (includes MB-M3 and MB-MP1 neurons).

(Aso et al., 2012)

ScerGAL4^Ddc.HL9 drives expression in 60 neurons of the dopaminergic cluster PAL, 3 neurons of the PAL, 3 neurons of the PPL2ab, 8 neurons of the PPM2 and 1 neuron of the PPM3 cluster. ScerGAL4^Ddc.HL9 labels more neurons of the PAM cluster than ScerGAL4^ple.PF.

(Claridge-Chang et al., 2009)

Marker for

Reflects expression of

Ddc

(Aso et al., 2012, Claridge-Chang et al., 2009)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | heat sensitive, with TrpA1^UAS.(B).cKa

(Keleman et al., 2012)

abnormal locomotor behavior | adult stage, with Fer2^RNAi.UAS.4

(Bou Dib et al., 2014)

abnormal locomotor behavior | adult stage, with Fer2^RNAi.UAS.5

(Bou Dib et al., 2014)

abnormal locomotor behavior | adult stage | heat sensitive, with Hsap\KCNJ2^UAS.EGFP

(Claridge-Chang et al., 2009)

abnormal locomotor behavior | adult stage | progressive, with Hsap\SNCA^A53T.UAS

(Alexopoulou et al., 2016)

abnormal memory | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Liu et al., 2012)

abnormal memory | heat sensitive, with TrpA1^UAS.(B).cKa

(Aso et al., 2012)

abnormal neuroanatomy, with Fer2^RNAi.UAS.5

(Bou Dib et al., 2014)

abnormal neurophysiology | progressive, with Hsap\LRRK2^{G2019S.UAS.Tag:FLAG}

(Hindle et al., 2013)

abnormal sleep | adult stage, with BTBD9^GD9543

(Freeman et al., 2012)

Phenotype Manifest In

dopaminergic PAM neuron, with Fer2^RNAi.UAS.5

(Bou Dib et al., 2014)

mitochondrion, with Fer2^RNAi.UAS.4

(Bou Dib et al., 2014)

Detailed Description

Statement

Reference

Inhibition (using shi^1.UAS at 32[o]C) of Scer\GAL4^Ddc.HL9 cells in adults does not affect 5 min (single-trial conditioned) aversive taste memory, compared to controls.

(Kirkhart and Scott, 2015)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of Scer\GAL4^Ddc.HL9 cells in adults, in combination with a paired odor, results in a significant increase in 2-min associative attractive olfactory memory, compared to controls.

(Liu et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\SNCA^A53T.UAS, Scer\GAL4^Ddc.HL9 has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible | partially by Usp8^HMS01898, Scer\GAL4^Ddc.HL9

(Alexopoulou et al., 2016)

Suppressor of

Statement

Reference

Usp8^HMS01898, Scer\GAL4^Ddc.HL9 is a suppressor | partially of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\SNCA^A53T.UAS, Scer\GAL4^Ddc.HL9

(Alexopoulou et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\SNCA^A53T.UAS, Scer\GAL4^Ddc.HL9 has dopaminergic PPM1 neuron | adult stage phenotype, suppressible | partially by Usp8^HMS01898/Scer\GAL4^GMR.PU

(Alexopoulou et al., 2016)

Hsap\SNCA^A53T.UAS, Scer\GAL4^Ddc.HL9 has dopaminergic PPM2 neuron | adult stage phenotype, suppressible | partially by Usp8^HMS01898/Scer\GAL4^GMR.PU

(Alexopoulou et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^Ddc.HL9/Fer2^UAS.Tag:FLAG fails to rescue Fer2^e03248

(Bou Dib et al., 2014)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^Ddc.HL9

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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