FB2024_03 , released June 25, 2024
Allele: Dmel\HUAS.cMb
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General Information
Symbol
Dmel\HUAS.cMb
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Maier
FlyBase ID
FBal0270104
Feature type
allele
Associated gene
Dmel\H
Associated Insertion(s)
Carried in Construct
M{UAS-H.Mb}
Also Known As
UAS-H, HFL
Key Links
Transgenic product class
wild_type
(Maier et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Maier et al., 2011)
Mutagen
in vitro construct
(Maier et al., 2011)
Progenitor genotype
Carried in construct
M{UAS-H.Mb}
Cytology
Description

UAS regulatory sequences drive expression of H.

(Maier et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
H
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing HUAS.cMb under the control of Scer\GAL4GMR.PU results in slightly smaller eyes with a rough appearance, fused or collapsed ommatidia and ectopic or absent interommatidial bristles.

      Expressing HUAS.cMb under the control of Scer\GAL4sd.PU leads to severely reduced wing area, strong incisions of the wing margin at the edges and the tip, vein thickening and ectopic nets of veins at the distal part of the longitudinal veins L2 and L5 when flies are raised at 18[o]C. At a rearing temperature of 25[o]C, wings are present only as severely malformed and reduced rudiments. Under the control of Scer\GAL4bi.PU, wings are significantly smaller, with incisions of the wing margin at the tip, vein thickening and ectopic veins at the distal part of L2 and L5 (but no ectopic bristles) at 18[o]C, and more severely at 25[o]C.

      (Smylla et al., 2019)

      Scer\GAL4Bx-MS1096-mediated expression of HScer\UAS.cMb causes a transformation of sockets into shafts (mostly affecting microchaetae) as well as outer into inner cell fates during bristle development, resulting in double shafts and bald cuticle.

      (Maier et al., 2013)

      Expression of HScer\UAS.attB.cMa under the control of Scer\GAL4bi-omb-Gal4 results in wing disc tissue loss due to cell death.

      Expression of HScer\UAS.attB.cMa under the control of Scer\GAL4GMR.PF results in small irregular eyes.

      (Kurth et al., 2011)

      Expression of HScer\UAS.attB.cMa under the control of Scer\GAL4Bx-MS1096 results in bristle loss and socket to shaft transformations, mainly of the macrochaetae.

      (Maier et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      HUAS.cMb, Scer\GAL4GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4GMR.PF, Su(H)UAS.cMa

      (Kurth et al., 2011)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expressing Su(H)UAS.cMa with HUAS.cMb under the control of Scer\GAL4sd.PU results in rudimentary wings and ectopic bristle tufts mainly on the scutellum when flies are raised at 18[o]C, and complete wing loss with more scutellar bristles at 25[o]C. Adults are barely viable and need assistance at eclosion from the pupal case.

      (Smylla et al., 2019)

      Scer\GAL4Bx-MS1096-mediated co-expression of HScer\UAS.cMb and Su(H)Scer\UAS.cMa strongly affects all N-dependent steps of bristle formation - asymmetric cell divisions as well as lateral inhibition. Bald patches, double bristles and bristle clusters are visible on the head and thorax. The eye is almost completely absent without any visible external structures. Flies die as pharate adults.

      Scer\GAL4Bx-MS1096-mediated co-expression of HScer\UAS.cMb and Su(H)CTD.T:Hsap\MYC results in mild phenotypes and flies hatch normally. Bristle development is subtly disturbed - double sockets are occasionally seen, as are bristle loss and a slightly higher bristle density.

      Scer\GAL4bi-omb-Gal4-mediated co-expression of HScer\UAS.cMb and Su(H)Scer\UAS.cMa leads to a very small wing disc, whereas co-expression of HScer\UAS.cMb and Su(H)CTD.T:Hsap\MYC has almost no influence on disc size.

      (Maier et al., 2013)

      Expression of Su(H)Scer\UAS.cMa enhances the wing disc tissue loss seen when HScer\UAS.attB.cMa is expressed under the control of Scer\GAL4bi-omb-Gal4.

      Expression of Su(H)WARE.Scer\UAS enhances the wing disc tissue loss seen when HScer\UAS.attB.cMa is expressed under the control of Scer\GAL4bi-omb-Gal4.

      Expression of Su(H)Scer\UAS.cMa enhances the eye phenotype seen when HScer\UAS.attB.cMa is expressed under the control of Scer\GAL4GMR.PF. Only small slits lacking ommatidial structures and eye colour remain.

      Expression of Su(H)WARE.Scer\UAS enhances the eye phenotype seen when HScer\UAS.attB.cMa is expressed under the control of Scer\GAL4GMR.PF. Only small slits lacking ommatidial structures and eye colour remain.

      (Kurth et al., 2011)

      Co-expression of HScer\UAS.attB.cMa and Su(H)Scer\UAS.cMa under the control of Scer\GAL4bi-omb-Gal4 results in severely disturbed growth and development of the third instar larval wing disc.

      Expression of Su(H)Scer\UAS.cMa strongly enhances the bristle loss phenotype seen when HScer\UAS.attB.cMa is expressed under the control of Scer\GAL4Bx-MS1096. Bushes of bristles appear as a result of reduced lateral inhibition.

      (Maier et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      HScer\UAS.attB.cMa
      HScer\UAS.cMb
      HUAS.cMb
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Maier
      Secondary FlyBase IDs
        References (9)