FlyBase Allele Report: Dmel\H[LD.UAS]

General Information

Symbol

Dmel\H^LD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0270105

Feature type

allele

Associated gene

Dmel\H

Associated Insertion(s)

Carried in Construct

M{UAS-H.LD}

Also Known As

H^LD, UAS-H^LD

Key Links

Transgenic product class

missense_variant

(Maier et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Maier et al., 2011)

Mutagen

in vitro construct

(Maier et al., 2011)

Progenitor genotype

H^UAS.cMb

(Maier et al., 2011)

Carried in construct

M{UAS-H.LD}

Cytology

Description

UAS regulatory sequences drive expression of a mutant form of H in which the leucine at residue 235 has been replaced by aspartic acid.

(Maier et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^bi.PU

(Smylla et al., 2019)

abnormal size | adult stage, with Scer\GAL4^GMR.PU

(Smylla et al., 2019)

abnormal size | adult stage, with Scer\GAL4^sd.PU

(Smylla et al., 2019)

decreased size | adult stage, with Scer\GAL4^bi-omb-Gal4

(Nagel et al., 2020)

visible | adult stage, with Scer\GAL4^bi.PU

(Smylla et al., 2019)

visible | adult stage, with Scer\GAL4^bi-omb-Gal4

(Nagel et al., 2020)

visible | adult stage, with Scer\GAL4^GMR.PU

(Smylla et al., 2019)

visible | adult stage, with Scer\GAL4^sd.PU

(Smylla et al., 2019)

visible | adult stage, with Scer\GAL4^vg.QE

(Nagel et al., 2020)

Phenotype Manifest In

chaeta | ectopic, with Scer\GAL4^sd.PU

(Smylla et al., 2019)

eye, with Scer\GAL4^GMR.PU

(Smylla et al., 2019)

interommatidial bristle, with Scer\GAL4^GMR.PU

(Smylla et al., 2019)

interommatidial bristle | ectopic, with Scer\GAL4^GMR.PU

(Smylla et al., 2019)

wing, with Scer\GAL4^bi.PU

(Smylla et al., 2019)

wing, with Scer\GAL4^bi-omb-Gal4

(Nagel et al., 2020)

wing, with Scer\GAL4^sd.PU

(Smylla et al., 2019)

wing margin, with Scer\GAL4^sd.PU

(Smylla et al., 2019)

wing vein | ectopic, with Scer\GAL4^bi.PU

(Smylla et al., 2019)

wing vein | ectopic, with Scer\GAL4^bi-omb-Gal4

(Nagel et al., 2020)

wing vein | ectopic, with Scer\GAL4^sd.PU

(Smylla et al., 2019)

wing vein | ectopic, with Scer\GAL4^vg.QE

(Nagel et al., 2020)

Detailed Description

Statement

Reference

Expressing H^LD.UAS under the control of Scer\GAL4^GMR.PU results in slightly smaller eyes with a rough appearance and ectopic or absent interommatidial bristles.

Expressing H^LD.UAS under the control of Scer\GAL4^sd.PU leads to overall reduced wing area, vein thickening and ectopic nets of veins at the distal part of the longitudinal veins L2 and L5 when flies are raised at 18[o]C. At a rearing temperature of 25[o]C, wings display a further area reduction, incisions of the wing margin at the tip and ectopic bristles (chaetae) along ectopic veins. Under the control of Scer\GAL4^bi.PU, wings are significantly smaller and display ectopic veins at the distal part of L2 and L5 (but no incisions or ectopic bristles) at 18[o]C, and more severely at 25[o]C.

(Smylla et al., 2019)

Expression of H^LD.Scer\UAS under the control of Scer\GAL4^bi-omb-Gal4 has no effect on wing disc proliferation.

Expression of H^LD.Scer\UAS under the control of Scer\GAL4^GMR.PF has no effect on wing disc proliferation.

(Kurth et al., 2011)

Expression of H^LD.Scer\UAS under the control of Su(H)^Scer\UAS.cMa has very little effect on the bristles.

(Maier et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

H^LD.UAS, Scer\GAL4^sd.PU is a non-suppressor of lethal - all die during pharate adult stage phenotype of Scer\GAL4^sd.PU, Su(H)^UAS.cMa

(Smylla et al., 2019)

H^LD.UAS, Scer\GAL4^sd.PU is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^sd.PU, Su(H)^UAS.cMa

(Smylla et al., 2019)

H^LD.UAS, Scer\GAL4^sd.PU is a non-suppressor of abnormal size | pharate adult stage phenotype of Scer\GAL4^sd.PU, Su(H)^UAS.cMa

(Smylla et al., 2019)

H^LD.UAS, Scer\GAL4^bi-omb-Gal4 is a non-suppressor of hyperplasia | third instar larval stage phenotype of Scer\GAL4^bi-omb-Gal4, Su(H)^WARE.UAS

(Kurth et al., 2011)

H^LD.UAS, Scer\GAL4^GMR.PF is a non-suppressor of hyperplasia | adult stage phenotype of Scer\GAL4^GMR.PF, Su(H)^WARE.UAS

(Kurth et al., 2011)

H^LD.UAS, Scer\GAL4^GMR.PF is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, Su(H)^WARE.UAS

(Kurth et al., 2011)

Other

Statement

Reference

H^LD.UAS, Scer\GAL4^vg.QE, Su(H)^UAS.cMa has visible | adult stage phenotype

(Nagel et al., 2020)

H^LD.UAS, Scer\GAL4^bi-omb-Gal4, Su(H)^UAS.cMa has viable phenotype

(Nagel et al., 2020)

H^LD.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has visible | adult stage phenotype

(Smylla et al., 2019)

H^LD.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has abnormal size | adult stage phenotype

(Smylla et al., 2019)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

H^LD.UAS, Scer\GAL4^Bx-MS1096 is a non-enhancer of macrochaeta phenotype of Scer\GAL4^Bx-MS1096, Su(H)^UAS.cMa

(Maier et al., 2011)

H^LD.UAS, Scer\GAL4^Bx-MS1096 is a non-enhancer of microchaeta phenotype of Scer\GAL4^Bx-MS1096, Su(H)^UAS.cMa

(Maier et al., 2011)

NOT Suppressor of

Statement

Reference

H^LD.UAS, Scer\GAL4^sd.PU is a non-suppressor of wing | pharate adult stage phenotype of Scer\GAL4^sd.PU, Su(H)^UAS.cMa

(Smylla et al., 2019)

H^LD.UAS, Scer\GAL4^bi-omb-Gal4 is a non-suppressor of wing disc | third instar larval stage phenotype of Scer\GAL4^bi-omb-Gal4, Su(H)^WARE.UAS

(Kurth et al., 2011)

H^LD.UAS, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, Su(H)^WARE.UAS

(Kurth et al., 2011)

Other

Statement

Reference

H^LD.UAS, Scer\GAL4^vg.QE, Su(H)^UAS.cMa has wing vein phenotype

(Nagel et al., 2020)

H^LD.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has wing phenotype

(Smylla et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing Su(H)^UAS.cMa with H^LD.UAS under the control of Scer\GAL4^sd.PU results in enlarged and malformed wings when flies are raised at 18[o]C, and more severely at 25[o]C.

(Smylla et al., 2019)

Expression of H^LD.Scer\UAS does not suppress the wing disc hyperproliferation phenotype seen when Su(H)^Scer\UAS.cMa is expressed under the control of Scer\GAL4^bi-omb-Gal4.

Expression of H^LD.Scer\UAS does not suppress the wing disc hyperproliferation phenotype seen when Su(H)^{WARE.Scer\UAS} is expressed under the control of Scer\GAL4^bi-omb-Gal4.

Expression of H^LD.Scer\UAS does not suppress the eye hyperproliferation phenotype seen when Su(H)^Scer\UAS.cMa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of H^LD.Scer\UAS does not suppress the eye hyperproliferation phenotype seen when Su(H)^{WARE.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

(Kurth et al., 2011)

Expression of H^LD.Scer\UAS does not enhance the wing disc proliferation defects seen when Su(H)^Scer\UAS.cMa is expressed under the control of Scer\GAL4^bi-omb-Gal4.

Expression of H^LD.Scer\UAS does not enhance the bristle phenotypes seen when Su(H)^Scer\UAS.cMa is expressed under the control of Scer\GAL4^Bx-MS1096.

(Maier et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

H^LD.Scer\UAS

H^LD.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

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