FlyBase Allele Report: Dmel\spidey[NIG.1444R]

General Information

Symbol

Dmel\spidey^NIG.1444R

Species

D. melanogaster

Name

FlyBase ID

FBal0271869

Feature type

allele

Associated gene

Dmel\spidey

Associated Insertion(s)

Carried in Construct

P{NIG.1444R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.1444R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-1444R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle | larval stage | nutrition conditional, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

abnormal starvation stress response | larval stage, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

abnormal starvation stress response | larval stage, with Scer\GAL4^NP6073, Scer\GAL80^elav.PU

(Cinnamon et al., 2016)

increased cell size | larval stage, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

Phenotype Manifest In

embryonic/larval oenocyte | larval stage, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

embryonic/larval oenocyte | larval stage, with Scer\GAL4^NP6073, Scer\GAL80^elav.PU

(Cinnamon et al., 2016)

embryonic/larval tracheal section | larval stage, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

nucleus | larval stage | nutrition conditional, with Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

Detailed Description

Statement

Reference

Somatic clones in the larval fat body expressing spidey^NIG.1444R under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} do not display a detectable change in lipid droplets or cell size during starvation, as compared to controls.

An increased proportion of larvae expressing spidey^NIG.1444R under the control of Scer\GAL4^Desat1.PB display tracheal flooding, a significant increase in larval oenocyte size in both fed conditions at 48 hours after hatching, and starved conditions 66 hours after hatching, but there is no difference in oenocyte size during fed conditions 66 hours after hatching, as compared to controls. Oenocyte enlargement during starvation is also associated with enlarged nuclei(Fig. 5A), and during starvation, oenocyte nuclei undergo higher levels of endoreplication, as compared to controls (5B).

Expression of spidey^NIG.1444R under the control of Scer\GAL4^Desat1.PB, or under the control of Scer\GAL4^{Cyp4g1-NP6073} and Scer\GAL80^elav.PU to restrict expression to oenocytes, results in an increase in the accumulation of lipid droplets in larval oenocytes during fed conditions, but a decrease in the accumulation of lipid droplets in larval oenocytes during starvation, as compared to controls.

(Cinnamon et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^Desat1.PB, spidey^NIG.1444R has abnormal starvation stress response | larval stage phenotype, non-enhanceable by Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^Desat1.PB, spidey^NIG.1444R has increased cell size | larval stage phenotype, suppressible | partially by Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^Desat1.PB, spidey^NIG.1444R has abnormal starvation stress response | larval stage phenotype, non-suppressible by Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^Desat1.PB, spidey^NIG.1444R has embryonic/larval oenocyte | larval stage phenotype, non-enhanceable by Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^Desat1.PB, spidey^NIG.1444R has embryonic/larval oenocyte | larval stage phenotype, suppressible | partially by Pi3K92E^A2860C.UAS, Scer\GAL4^Desat1.PB

(Cinnamon et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Pi3K92E^{A2860C.Scer\UAS} does not enhance or suppress the increase in lipid droplets in larval oenocytes seen in starved larvae, but it partially suppresses the increased oenocyte size in both fed and starved larvae expressing spidey^NIG.1444R under the control of Scer\GAL4^Desat1.PB.

(Cinnamon et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG1444^1444R-2

(Cinnamon et al., 2016)

CG1444^NIG.1444R

spidey^NIG.1444R

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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