FlyBase Allele Report: Dmel\Usp30[NIG.3016R]

General Information

Symbol

Dmel\Usp30^NIG.3016R

Species

D. melanogaster

Name

FlyBase ID

FBal0273294

Feature type

allele

Associated gene

Dmel\Usp30

Associated Insertion(s)

Carried in Construct

P{NIG.3016R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.3016R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-3016R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by park²⁵

(Bingol et al., 2014)

modeled by Pink1^B9

(Bingol et al., 2014)

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-PR^PO-36.UAS

(Park et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Unlike controls, flies expressing USP30^NIG.3016R via Scer\GAL4^Ddc.PL, Scer\GAL4^Act5C.PI or Scer\GAL4^ple.PF show no impairment in climbing performance following treatment with paraquat.

Flies expressing USP30^NIG.3016R via Scer\GAL4^Ddc.PL, Scer\GAL4^Act5C.PI or Scer\GAL4^ple.PF that are fed with paraquat live significantly longer than controls fed with paraquat.

(Bingol et al., 2014)

Expression of CG3016^NIG.3016R under the control of Scer\GAL4^bbg-C96 does not result in a wing phenotype.

Expression of CG3016^NIG.3016R under the control of Scer\GAL4^dpp.PU does not result in a bristle phenotype.

(Zhang et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Ddc.PL, Usp30^NIG.3016R has abnormal oxidative stress response | chemical conditional phenotype, suppressible by Hsap\USP30^UAS.cBa, Scer\GAL4^Ddc.PL

(Bingol et al., 2014)

Scer\GAL4^Act5C.PI, Usp30^NIG.3016R has abnormal oxidative stress response | chemical conditional phenotype, suppressible by Hsap\USP30^UAS.cBa, Scer\GAL4^Act5C.PI

(Bingol et al., 2014)

Scer\GAL4^Ddc.PL, Usp30^NIG.3016R has chemical resistant phenotype, suppressible by Hsap\USP30^UAS.cBa, Scer\GAL4^Ddc.PL

(Bingol et al., 2014)

Scer\GAL4^Act5C.PI, Usp30^NIG.3016R has chemical resistant phenotype, suppressible by Hsap\USP30^UAS.cBa, Scer\GAL4^Act5C.PI

(Bingol et al., 2014)

Suppressor of

Statement

Reference

Usp30^NIG.3016R/Scer\GAL4^Act5C.PI is a suppressor of abnormal locomotor behavior phenotype of Pink1^B9

(Bingol et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Usp30^NIG.3016R/Scer\GAL4^Act5C.PI is a suppressor of mitochondrion phenotype of Pink1^B9

(Bingol et al., 2014)

Usp30^NIG.3016R/Scer\GAL4^Act5C.PI is a suppressor of mitochondrial crista phenotype of Pink1^B9

(Bingol et al., 2014)

Usp30^NIG.3016R/Scer\GAL4^Act5C.PI is a suppressor of adult brain phenotype of Pink1^B9

(Bingol et al., 2014)

Usp30^NIG.3016R/Scer\GAL4^Act5C.PI is a suppressor of mitochondrion phenotype of park²⁵

(Bingol et al., 2014)

Usp30^NIG.3016R/Scer\GAL4^Act5C.PI is a suppressor of mitochondrial crista phenotype of park²⁵

(Bingol et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^Act5C.PI-mediated expression of USP30^NIG.3016R largely restores the park²⁵ or Pink1^B9 mitochondrial morphology defects.

Scer\GAL4^Act5C.PI-mediated expression of USP30^NIG.3016R ameliorates the climbing defect and prevents the dopamine depletion seen in Pink1^B9 flies.

(Bingol et al., 2014)

Xenogenetic Interactions

Statement

Reference

The benefit provided by Scer\GAL4^Act5C.PI- or Scer\GAL4^Ddc.PL-mediated expression of USP30^NIG.3016R in paraquat-treated flies is reversed by co-expression of RNAi-resistant Hsap\USP30^Scer\UAS.cBa in both climbing and survival assays.

(Bingol et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG3016^NIG.3016R

USP30^NIG.3016R

Usp30^NIG.3016R

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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