FlyBase Allele Report: Dmel\lark[RNAi.UAS.cSb]

General Information

Symbol

Dmel\lark^RNAi.UAS.cSb

Species

D. melanogaster

Name

FlyBase ID

FBal0277027

Feature type

allele

Associated gene

Dmel\lark

Associated Insertion(s)

Carried in Construct

P{UAS-lark.RNAi.Sb}

Key Links

Transgenic product class

RNAi_reagent

(Sundram et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Sundram et al., 2012)

Mutagen

in vitro construct

(Sundram et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-lark.RNAi.Sb}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat containing 76bp of lark coding sequence and the 3'UTR.

(Sundram et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

lark

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eclosion rhythm, with Scer\GAL4^phtm.PO

(Sundram et al., 2012)

abnormal eclosion rhythm, with Scer\GAL4^tim.UAS

(Sundram et al., 2012)

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf, lark¹

(Huang et al., 2014)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^dimm-929

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^dimm-929, lark¹

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^elav-C155

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^elav-C155, lark¹

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^Mai179

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^Mai179, lark¹

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^nrv2.PS

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^nrv2.PS, lark¹

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^P2.4.Pdf, lark¹

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^tim.UAS

(Sundram et al., 2012)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^tim.UAS, lark¹

(Sundram et al., 2012)

lethal - all die before end of pupal stage, with Scer\GAL4^Act5C.PU

(Sundram et al., 2012)

some die during pupal stage, with Scer\GAL4^Act5C.PU

(Sundram et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of lark^{dsRNA.Scer\UAS.cSb} driven by Scer\GAL4^P2.4.Pdf in a lark¹/+ background results in a significant shortening of circadian period.

(Huang et al., 2014)

No defects in locomotor rhythm are observed when flies expressing lark^{dsRNA.Scer\UAS.cSb} under the control of Scer\GAL4^elav-C155 are raised at 25[o]C. However, when the flies are reared at 29[o]C, 21% of flies are arrhythmic and 16% are weakly arrhythmic. Heterozygous lark¹/+ enhances the phenotype further.

No defects in locomotor rhythmicity are observed when flies expressing lark^{dsRNA.Scer\UAS.cSb} under the control of Scer\GAL4^nrv2.PS are raised at 25[o]C. When the flies are reared at 29[o]C, 20% of flies are arrhythmic and 35% are weakly arrhythmic. Heterozygous lark¹/+ enhances the phenotype further; 42% of these flies are arrhythmic.

Only 21% of flies expressing two copies of lark^{dsRNA.Scer\UAS.cSb} under the control of Scer\GAL4^tim.Scer\UAS exhibit robust locomotor rhythms at 29[o]C. This rises to 57% when only one copy of the transgene is expressed. Heterozygous lark¹/+ enhances the phenotype further; 51% of these flies are completely arrhythmic and a further 29% are weakly arrhythmic.

Only 12% of flies expressing two copies of lark^{dsRNA.Scer\UAS.cSb} under the control of Scer\GAL4^P2.4.Pdf (with Dcr-2^Scer\UAS.cDa) in a heterozygous lark¹ background display robust locomotor rhythms. 37% of flies are arrhythmic.

48% of flies expressing lark^{dsRNA.Scer\UAS.cSb} under the control of Scer\GAL4^dimm-929 at 29[o]C exhibit robust locomotor rhythms. The proportion of arrhythmic flies increases when knockdown occurs in a lark¹/+ mutant background.

40% of flies expressing lark^{dsRNA.Scer\UAS.cSb} under the control of Scer\GAL4^Mai179 at 29[o]C exhibit robust locomotor rhythms. The proportion of arrhythmic flies increases when knockdown occurs in a lark¹/+ mutant background.

Only 52% of flies expressing lark^{dsRNA.Scer\UAS.cSb} under the control of Scer\GAL4^dimm-929 (restricted to non-pdf expressing cell types using Scer\GAL80^Pdf.PS) in a lark¹/+ mutant background at 29[o]C exhibit strong locomotor rhythm defects.

Strong locomotor defects are seen in 56% of flies expressing lark^{dsRNA.Scer\UAS.cSb} under the control of Scer\GAL4^Mai179 (restricted to non-pdf expressing cell types using Scer\GAL80^Pdf.PS) in a lark¹/+ mutant background and raised at 29[o]C.

Flies expressing lark^{dsRNA.Scer\UAS.cSb} under the control of any of Scer\GAL4^elav-C155, Scer\GAL4^Ccap.PP, Scer\GAL4^Eh.2.4, Scer\GAL4^dimm-929, Scer\GAL4^P2.4.Pdf or Scer\GAL4^tim.Scer\UAS show normal daily eclosion profiles in LD (12hours light: 12 hours dark) or DD (constant darkness). Elevated nighttime eclosion is seen when lark^{dsRNA.Scer\UAS.cSb} is expressed under the control of Scer\GAL4^tim.Scer\UAS.

Flies expressing lark^{dsRNA.Scer\UAS.cSb} under the control of Scer\GAL4^Mai60 show normal daily eclosion profiles.

Flies expressing lark^{dsRNA.Scer\UAS.cSb} under the control of Scer\GAL4^phm.PO display arrhythmic eclosion profiles in DD conditions (constant darkness), although variability is seen across experiments. Co-expressing Dcr-2^Scer\UAS.cDa reduces variability and all flies show weakly rhythmic eclosion profiles. Adult locomotor rhythm is unaffected.

(Sundram et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^P2.4.Pdf, lark¹, lark^RNAi.UAS.cSb has abnormal locomotor rhythm phenotype, suppressible | partially by dco^UAS.cKa/Scer\GAL4^P2.4.Pdf

(Huang et al., 2014)

Other

Statement

Reference

Scer\GAL4^P2.4.Pdf, dco^L, lark^RNAi.UAS.cSb has abnormal locomotor rhythm phenotype

(Huang et al., 2014)

Scer\GAL4^P2.4.Pdf, dco^S, lark^RNAi.UAS.cSb has abnormal locomotor rhythm phenotype

(Huang et al., 2014)

Scer\GAL4^P2.4.Pdf, dco^ar, lark^RNAi.UAS.cSb has abnormal locomotor rhythm phenotype

(Huang et al., 2014)

Scer\GAL4^P2.4.Pdf, dco^j3B9, lark^RNAi.UAS.cSb has abnormal locomotor rhythm phenotype

(Huang et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of dco^Scer\UAS.cKa significantly suppresses circadian rhythm defects in flies with lark^{dsRNA.Scer\UAS.cSb} driven by Scer\GAL4^P2.4.Pdf in a lark¹/+ background.

(Huang et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

lark^RNAi.UAS.cSb/lark¹ is rescued by lark^UAS.Tag:HA/Scer\GAL4^P2.4.Pdf

(Huang et al., 2014)

Comments

Co-expression of lark^{Scer\UAS.T:Ivir\HA1} rescues circadian rhythm defects in flies with lark^{dsRNA.Scer\UAS.cSb} driven by Scer\GAL4^P2.4.Pdf in a lark¹/+ background.

(Huang et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

lark^RNAi.UAS.cSb

lark^{dsRNA.Scer\UAS.cSb}

lark^{dsRNA.UAS.cSb}

Name Synonyms

Secondary FlyBase IDs

References (3)

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