FB2024_03 , released June 25, 2024
Allele: Dmel\psidinUAS.cKa
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General Information
Symbol
Dmel\psidinUAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kim
FlyBase ID
FBal0277251
Feature type
allele
Associated gene
Dmel\psidin
Associated Insertion(s)
Carried in Construct
P{UAS-psid.K}
Key Links
Transgenic product class
cDNA
(Kim et al., 2011)
wild_type
(Kim et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Kim et al., 2011)
wild_type
(Kim et al., 2011)
Mutagen
in vitro construct
(Kim et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-psid.K}
Cytology
Description

UAS regulatory sequences drive expression of the psidin AT25164 cDNA.

(Kim et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
psidin
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Border follicle cells expressing psidinScer\UAS.cKa under the control of Scer\GAL4slbo.2.6 at 29[o]C have longer actin-rich protrusions than control cells. The protrusions are highly irregular in morphology, as they are elongated, irregular in length, of variable thickness and can even be branched.

      (Kim et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Act5Cslbo.T:Avic\GFP-EGFP and of psidinScer\UAS.cKa under the control of Scer\GAL4slbo.2.6 results in a strong inhibition of border follicle cell migration.

      Expression of psidinScer\UAS.cKa under the control of Scer\GAL4slbo.2.6 in a tsr1, ssh1-63, WASp3, HemJ4-48 or zip1 heterozygous background has little or no effect on border cell migration.

      Expression of psidinScer\UAS.cKa under the control of Scer\GAL4slbo.2.6 in a Rac1J11 Rac2Δ double heterozygous background has little or no effect on border cell migration.

      (Kim et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      psidinUAS.cKa/Scer\GAL4Act.PU rescues psidin85D1/psidin55D4

      (Kim et al., 2011)

      psidinUAS.cKa/Scer\GAL4slbo.2.6 rescues psidin55D4

      (Kim et al., 2011)
      Comments

      Expression of psidinScer\UAS.cKa under the control of Scer\GAL4slbo.2.6 rescues the migration defects seen in homozygous psidin55D4 border cells.

      Expression of psidinScer\UAS.cKa under the control of Scer\GAL4Act.PU rescues the lethality of psidin55D4/psidin85D1 animals.

      (Kim et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      psidinScer\UAS.cKa
      psidinUAS.cKa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Kim
      Secondary FlyBase IDs
        References (2)