FlyBase Allele Report: Dmel\Tbcb[1]

General Information

Symbol

Dmel\Tbcb¹

Species

D. melanogaster

Name

FlyBase ID

FBal0277883

Feature type

allele

Associated gene

Dmel\Tbcb

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Baffet et al., 2012)

amorphic allele - molecular evidence

(Baffet et al., 2012)

Mutagen

ethyl methanesulfonate

(Baffet et al., 2012)

Progenitor genotype

Cytology

Description

Amino acid replacement: L87Q.

(Baffet et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:19,486,404..19,486,404 [-]

Nucleotide change:

T19486404A

Amino acid change:

L87Q | TBCB-PA

Reported amino acid change:

L87Q

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Baffet et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN | cell autonomous | somatic clone

(Okumura et al., 2015)

follicle cell | somatic clone

(Baffet et al., 2012)

neuroblast | larval stage

(Baffet et al., 2012)

nurse cell | somatic clone

(Baffet et al., 2012)

oocyte | germline clone

(Baffet et al., 2012)

Detailed Description

Statement

Reference

TBCB¹ mutant DL1 olfactory projection neuron clones exhibit ectopic dendrite arborization. Loss of dorsal branches in axons is seen at low penetrance.

(Okumura et al., 2015)

Significantly fewer microtubules are seen in TBCB¹ mutant oocytes and nurse cell clones throughout oogenesis compared to controls, although a few microtubules remain. The interphasic microtubule network in the epithelial follicle cells is also strongly depleted. Actin cytoskeleton is unaffected.

Clonal TBCB¹ mutant cystoblasts always divide correctly into 16 cell germline cysts, although microtubule defects are detected, including in the germarium. No proliferation defects are observed in follicle cell clones.

In third instar larval TBCB¹ homozygous mutant brains the mitotic index is similar to control brains.

In third instar larval TBCB¹/Df(2R)ED3728 mutant brains the mitotic index is similar to control brains.

Homozygous TBCB¹ mutant neuroblasts cultured from larvae are able to form mitotic spindles and are able to divide. However cell cycle progression is delayed compared to heterozygous control cells; 65% of cells are delayed in interphase as well as mitosis. The remaining cells are either arrested in metaphase or anaphase.

The antero-dorsal positioning of the nucleus is impaired in the TBCB¹ mutant stage 9-10 oocyte clones. However at stage 4 the process of oocyte differentiation appears to be unaffected. Large TBCB¹ mutant follicle cell clones form a disorganised multistratified epithelium, consistent with cell-polarity defects.

(Baffet et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^GH146, Tbcb¹, Tbcd^RNAi.UAS.cOa has cell lethal | somatic clone | cell autonomous phenotype

(Okumura et al., 2015)

αTub84B⁵, TBCB[+]/Tbcb¹ has abnormal cell polarity | recessive | adult stage phenotype

(Baffet et al., 2012)

αTub84B⁵, TBCB[+]/Tbcb¹ has semi-fertile | recessive phenotype

(Baffet et al., 2012)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^GH146, Tbcb¹, Tbcd^RNAi.UAS.cOa has adult antennal lobe projection neuron DL1 adPN | somatic clone | cell autonomous phenotype

(Okumura et al., 2015)

αTub84B⁵, TBCB[+]/Tbcb¹ has follicle cell phenotype

(Baffet et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Very few single-cell clones are obtained when TBCD^{miRNA.Scer\UAS} is expressed in TBCB¹ mutant DL1 olfactory projection neuron clones under the control of Scer\GAL4^GH146.

(Okumura et al., 2015)

Flies double heterozygous for αTub84B⁵ and TBCB¹ are viable, but display strongly reduced fertility and follicle cell polarity defects.

par-1^W3 complements TBCB¹.

(Baffet et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Tbcb¹ is rescued by Tbcb^+tBa

(Baffet et al., 2012)

Tbcb¹ is rescued by Tbcb^Ubi.EGFP

(Baffet et al., 2012)

Comments

Expression of TBCB^+tBa rescues the lethality seen in TBCB¹ mutant larvae.

Expression of TBCB^{Ubi.T:Avic\GFP-EGFP} rescues the lethality seen in TBCB¹ mutant larvae. The microtubule and cell polarity defects seen in TBCB¹ mutant clones are also rescued.

(Baffet et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

3.112

(Baffet et al., 2012)

TBCB¹

(Okumura et al., 2015)

Tbcb¹

dTBCB¹

(Baffet et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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