FlyBase Allele Report: Dmel\shrb[HMS01767]

General Information

Symbol

Dmel\shrb^HMS01767

Species

D. melanogaster

Name

FlyBase ID

FBal0278885

Feature type

allele

Associated gene

Dmel\shrb

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01767}

Also Known As

HMS01767, shrub RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01767}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01767

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis, with Scer\GAL4^nanos.PG

(Eikenes et al., 2015, Matias et al., 2015)

abnormal mitotic cell cycle | P-stage, with Scer\GAL4^sca.PU

(Bruelle et al., 2023)

majority die during embryonic stage | paternal effect, with Scer\GAL4^{VP16.nanos.UTR}

(Chen et al., 2021)

partially lethal - majority die | paternal effect, with Scer\GAL4^{VP16.nanos.UTR}

(Chen et al., 2021)

Phenotype Manifest In

cystic bulge | decreased number, with Scer\GAL4^{VP16.nanos.UTR}

(Chen et al., 2021)

egg chamber, with Scer\GAL4^nanos.PG

(Matias et al., 2015)

egg chamber, with Scer\GAL4^nanos.PG, Scer\GAL80^bam.PM

(Matias et al., 2015)

external sensory organ precursor cell | P-stage, with Scer\GAL4^sca.PU

(Bruelle et al., 2023)

female germline cell, with Scer\GAL4^nanos.PG

(Eikenes et al., 2015, Matias et al., 2015)

female germline cell, with Scer\GAL4^nanos.PG, Scer\GAL80^bam.PM

(Matias et al., 2015)

female germline stem cell, with Scer\GAL4^nanos.PG

(Eikenes et al., 2015, Matias et al., 2015)

midbody | P-stage, with Scer\GAL4^sca.PU

(Bruelle et al., 2023)

spermatozoon | absent, with Scer\GAL4^{VP16.nanos.UTR}

(Chen et al., 2021)

testis, with Scer\GAL4^{VP16.nanos.UTR}

(Chen et al., 2021)

waste bag | absent, with Scer\GAL4^{VP16.nanos.UTR}

(Chen et al., 2021)

Detailed Description

Statement

Reference

Expression of shrb^HMS01767 under the control of Scer\GAL4^nos.PG results in a number of defects in the female germline stem cells (GSCs). Approximately 45% of the GSCs form 'stem cysts' in which they are interconnected to several daughter cells (both linear and branched chains of cells are seen) and approximately 10% of the GSCs are polyploid. Approximately 50% of the egg chambers contain 32 germ cells.

(Eikenes et al., 2015)

More than 50% of egg chambers contain 32 cells in females expressing shrb^HMS01767 under the control of Scer\GAL4^nos.PG. In these egg chambers, the oocyte is linked to the nurse cells by 5 ring canals. Approximately 50% of germaria contain 'stem cysts' in which the female germline stem cells are interconnected to daughter cells. The cells in these stem cysts undergo synchronous division.

Egg chambers containing 32 cells are seen in females expressing shrb^HMS01767 under the control of Scer\GAL4^nos.PG in the presence of Scer\GAL80^bam.PM.

Females expressing shrb^HMS01767 under the control of Scer\GAL4^{bam.T:Hsim\VP16} have egg chambers containing the normal number of 16 cells.

(Matias et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38305

y¹ v¹; P{TRiP.HMS01767}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

shrb^HMS01767

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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