FlyBase Allele Report: Dmel\Dscam1[HMS01859]

General Information

Symbol

Dmel\Dscam1^HMS01859

Species

D. melanogaster

Name

FlyBase ID

FBal0278976

Feature type

allele

Associated gene

Dmel\Dscam1

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01859}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01859}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01859

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dendrite | embryonic stage, with Scer\GAL4^c544

(Kamiyama et al., 2015)

dendrite | embryonic stage, with Scer\GAL4^elav.PU, Scer\GAL80^eve.RN2

(Kamiyama et al., 2015)

dendrite | embryonic stage, with Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

dendrite | embryonic stage, with Scer\GAL4^ftz.ng

(Kamiyama et al., 2015)

dendrite | embryonic stage, with Scer\GAL4^GMR23E04

(Kamiyama et al., 2015)

dendrite | embryonic stage, with Scer\GAL4^GMR37G09

(Kamiyama et al., 2015)

larval DA1 motor neuron | embryonic stage, with Scer\GAL4^c544

(Kamiyama et al., 2015)

larval DA1 motor neuron | embryonic stage, with Scer\GAL4^elav.PU, Scer\GAL80^eve.RN2

(Kamiyama et al., 2015)

larval DA1 motor neuron | embryonic stage, with Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

larval DA1 motor neuron | embryonic stage, with Scer\GAL4^ftz.ng

(Kamiyama et al., 2015)

larval DA1 motor neuron | embryonic stage, with Scer\GAL4^GMR23E04

(Kamiyama et al., 2015)

larval DA1 motor neuron | embryonic stage, with Scer\GAL4^GMR37G09

(Kamiyama et al., 2015)

Detailed Description

Statement

Reference

Expression of Dscam1^HMS01859 under the control of Scer\GAL4^eve.RN2 results in defects in dendritogenesis in the embryonic aCC motoneurons (decreased number of dendritic tips, misplacement of primary branches) compared to controls. Loss of dendritic tips at the aCC neuron is also observed when the RNAi is driven with Scer\GAL4^elav.PU in combination with Scer\GAL80^eve.RN2 (thus excluding the expression from the aCC cells) or any of these drivers: Scer\GAL4[GMR23E04], Scer\GAL4^c544, Scer\GAL4^GMR37G09 or Scer\GAL4^ftz.ng but not when any of the following drivers are used: Scer\GAL4^GMR30C11, Scer\GAL4^GMR13G08, Scer\GAL4^GMR31E09, Scer\GAL4^GMR65G01, Scer\GAL4^GMR37G07, Scer\GAL4^GMR19H09, Scer\GAL4^GMR53D04, Scer\GAL4^GMR24B02, Scer\GAL4^sli.PS, Scer\GAL4^GMR34B10, Scer\GAL4^GMR30E02, Scer\GAL4^GMR32D08, Scer\GAL4^GMR55H07, Scer\GAL4^GMR22B04, Scer\GAL4^GMR11B11, Scer\GAL4^GMR27G12 and Scer\GAL4^GMR64F12. Both Scer\GAL4^GMR23E04 and Scer\GAL4^c544 drive expression in a random half of all MP1 neurons (but not in aCCs) and dendritogenesis defects are seen in aCC cells that run across an MP1 axon expressing the Dscam1^HMS01859 RNAi, no defects in the positioning of either the MP1 or the aCC neurons are observed in these embryos.

(Kamiyama et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

NOT suppressed by

Statement

Reference

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Pak^UAS.mEos2, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Cdc42⁴/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Cdc42⁶/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has larval DA1 motor neuron | embryonic stage phenotype, suppressible | partially by Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has dendrite | embryonic stage phenotype, suppressible | partially by Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

NOT suppressed by

Statement

Reference

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has larval DA1 motor neuron | embryonic stage phenotype, non-suppressible by Pak^UAS.mEos2, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has dendrite | embryonic stage phenotype, non-suppressible by Pak^UAS.mEos2, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has larval DA1 motor neuron | embryonic stage phenotype, non-suppressible by Cdc42⁴/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has dendrite | embryonic stage phenotype, non-suppressible by Cdc42⁴/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has larval DA1 motor neuron | embryonic stage phenotype, non-suppressible by Cdc42⁶/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Dscam1^HMS01859, Scer\GAL4^eve.RN2 has dendrite | embryonic stage phenotype, non-suppressible by Cdc42⁶/Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^eve.RN2

(Kamiyama et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The dendritogenesis defects in the embryonic aCC motoneurons characteristic for embryos expressing Dscam1^HMS01859 RNAi under the control of Scer\GAL4^eve.RN2 are partially suppressed by co-expression of Pak^{Scer\UAS.T:Myr-Src64B} : the reduced dendritic number is fully restored but the region containing primary dendritic processes is expanded compared to controls. This rescue effect is however blocked in the presence of either Cdc42⁶ or Cdc42⁴ (in hemizygous state). No suppression of the loss of dendritic tips is observed upon co-expression with Pak^{Scer\UAS.T:Lhem\EosFP-m2}.

(Kamiyama et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38945

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01859}attP2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Dscam1^HMS01859

Dscam^HMS01859

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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