FlyBase Allele Report: Dmel\Rcd-1r[GD17713]

General Information

Symbol

Dmel\Rcd-1r^GD17713

Species

D. melanogaster

Name

FlyBase ID

FBal0279795

Feature type

allele

Associated gene

Dmel\Rcd-1r

Associated Insertion(s)

Carried in Construct

P{GD17713}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Chen et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Chen et al., 2012)

Mutagen

in vitro construct

(Chen et al., 2012)

Progenitor genotype

Carried in construct

P{GD17713}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Chen et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD17713

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile, with Scer\GAL4^Act5C.PU

(Chen et al., 2012)

female fertile, with Scer\GAL4^Tub.PU

(Chen et al., 2012)

male semi-fertile, with Scer\GAL4^{VP16.nanos.UTR}

(Chen et al., 2012)

male semi-sterile, with Scer\GAL4^Act5C.PU

(Chen et al., 2012)

male semi-sterile, with Scer\GAL4^Tub.PU

(Chen et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

testis, with Scer\GAL4^Act5C.PU

(Chen et al., 2012)

Detailed Description

Statement

Reference

Expression of Rcd-1r^GD17713 under the control of either Scer\GAL4^Act5C.PU or Scer\GAL4^tub.PU results in an approximately 70% reduction in male fertility. Female fertility is unaffected.

Expression of Rcd-1r^GD17713 in the germline under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} produces male fertility defects, producing approximately 60% of the offspring in controls.

Expression of Rcd-1r^GD17713 in the accessory gland under the control of Scer\GAL4^Acp26Aa.PC has no effect on male fertility.

Testes from flies expressing Rcd-1r^GD17713 under the control of Scer\GAL4^Act5C.PU show specific defects in structure, including disorganised cysts, tumor formation and/or misoriented sperm bundles. Motile sperm are produced but they either fail to fertilize wild-type female eggs, or the fertilised eggs fail to develop.

(Chen et al., 2012)

Adults expressing Rcd-1r^GD17713 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Rcd-1r^GD17713, Scer\GAL4^{VP16.nanos.UTR} has male semi-fertile phenotype, non-suppressible by Rcd-1^UAS.cCa, Scer\GAL4^{VP16.nanos.UTR}

(Chen et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rcd-1^Scer\UAS.cCa fails to suppress the male fertility defects seen when Rcd-1r^GD17713 is expressed under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Chen et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v49821

w¹¹¹⁸ P{GD17713}v49821

v49820

w¹¹¹⁸; P{GD17713}v49820

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rcd-1r^GD17713

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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