A synthetic copy of the fs(1)K10 translation and localisation sequence (TLS), in which the A:U base pair at the 1st position of the TLS stem has been changed to G:C (minor groove mutation), has been inserted into engineered BglII and XbaI sites of fs(1)K10+t5.3.
fs(1)K101AU.GC rescues fs(1)K10LM00
fs(1)K101AU.GC rescues the egg hatching defect of fs(1)K10LM00 mutants.