A synthetic copy of the fs(1)K10 translation and localisation sequence (TLS), in which one of the base pairs that comprise the TLS stem has been reversed (major groove mutation), has been inserted into engineered BglII and XbaI sites of fs(1)K10+t5.3.
fs(1)K10rev5 rescues fs(1)K10LM00
fs(1)K10rev5 rescues the egg hatching defect of fs(1)K10LM00 mutants.