A synthetic copy of the fs(1)K10 translation and localisation sequence (TLS), in which several of the base pairs that comprise the TLS stem have been reversed (major groove mutation), has been inserted into engineered BglII and XbaI sites of fs(1)K10+t5.3.
fs(1)K10rev3-7 rescues fs(1)K10LM00
fs(1)K10rev3-7 rescues the egg hatching defect of fs(1)K10LM00 mutants.