A synthetic copy of the translation and localisation sequence (TLS), containing a 10 nucleotide loop, has been inserted into engineered BglII and XbaI sites of fs(1)K10+t5.3.
fs(1)K10loop10 fails to rescue fs(1)K10LM00
fs(1)K10loop10 does not rescue the egg hatching defect of fs(1)K10LM00 mutants.