FlyBase Allele Report: Dmel\mir-312[UAS.cSa]

General Information

Symbol

Dmel\mir-312^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Schertel

FlyBase ID

FBal0282233

Feature type

allele

Associated gene

Dmel\mir-312

Associated Insertion(s)

Carried in Construct

M{UAS-mir-312.S}

Key Links

Transgenic product class

wild_type

(Schertel et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Schertel et al., 2012)

Mutagen

in vitro construct

(Schertel et al., 2012)

Progenitor genotype

Carried in construct

M{UAS-mir-312.S}

Cytology

Description

UAS regulatory sequences drive expression of the mir-312 miRNA precursor sequence.

(Schertel et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-312

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Act5C.PU

(Schertel et al., 2012)

visible, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2012, Schertel et al., 2012)

Phenotype Manifest In

campaniform sensillum | ectopic, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2012)

wing, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2012, Schertel et al., 2012)

wing hair, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2012, Schertel et al., 2012)

wing sensillum | ectopic, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2012)

wing vein, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2012)

Detailed Description

Statement

Reference

Expression of mir-312^Scer\UAS.cSa under the control of Scer\GAL4^ey.PU does not result in a mutant phenotype in the eye.

Expression of mir-312^Scer\UAS.cSa under the control of Scer\GAL4^Bx-MS1096 results in curly wings with patches that lack wing hairs. The wing veins are thin in the posterior part of the wing and ectopic campaniform sensillae are seen.

(Schertel et al., 2012)

Expression of mir-312^Scer\UAS.cSa under the control of Scer\GAL4^Bx-MS1096 results in loss of wing hairs, deformation of the wing blade and formation of ectopic sensillae on the wing.

(Schertel et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, mir-312^UAS.cSa has visible phenotype, suppressible by sha^UAS.GFP/Scer\GAL4^Bx-MS1096

(Schertel et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, mir-312^UAS.cSa has wing hair phenotype, suppressible by sha^UAS.GFP/Scer\GAL4^Bx-MS1096

(Schertel et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of sha^{Scer\UAS.T:Avic\GFP} suppresses the loss of wing hairs caused by expression of mir-312^Scer\UAS.cSa under the control of Scer\GAL4^Bx-MS1096.

(Schertel et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

FlyORF

F002076

M{UAS-mir-312.S}ZH-86Fb

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mir-312^Scer\UAS.cSa

mir-312^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Schertel

Secondary FlyBase IDs

References (2)

Report Sections

Open Close