FB2024_03 , released June 25, 2024
Allele: Dmel\Hr4UAS.cQa
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General Information
Symbol
Dmel\Hr4UAS.cQa
Species
D. melanogaster
Name
FlyBase ID
FBal0282372
Feature type
allele
Associated gene
Dmel\Hr4
Associated Insertion(s)
Carried in Construct
P{UAS-Hr4.Q}
Key Links
Transgenic product class
cDNA
(Ou et al., 2011)
wild_type
(Ou et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Ou et al., 2011)
wild_type
(Ou et al., 2011)
Mutagen
in vitro construct
(Ou et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-Hr4.Q}
Cytology
Description

UAS regulatory sequences drive expression of a 6.3kb fragment containing the full-length synthetic cDNA of Hr4.

(Ou et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hr4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hr4Scer\UAS.cQa under the control of Scer\GAL4P0206 results in first instar larval arrest. These L1 larvae grow very large, accumulate lipids in their fat bodies, and have larger organs than controls due to continued proliferation. These flies also exhibit small ring glands compared to controls. This indicates that expression of Hr4 in the prothoracic glands specifically blocks molting and does not trigger lethality.

      Expression of Hr4Scer\UAS.cQa under the control of Scer\GAL4phm.PO results in second instar larval arrest. Prepupae form with an approximate 20 hour delay, compared with controls.

      Addition of 20E to the feeding medium rescues the lethality found upon expression of Hr4Scer\UAS.cQa under the control of Scer\GAL4P0206 or Scer\GAL4phm.PO.

      (Ou et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Hr4UAS.cQa, Scer\GAL4P0206 has ring gland phenotype, suppressible by Ras85DV12.UAS, Scer\GAL4P0206

      (Ou et al., 2011)
      Suppressor of
      Statement
      Reference

      Scer\GAL4P0206/Hr4UAS.cQa is a suppressor of prothoracic gland phenotype of Ras85DV12.UAS, Scer\GAL4phtm.PO

      (Ou et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Ras85DV12.Scer\UAS and Hr4Scer\UAS.cQa under the control of Scer\GAL4P0206 results in wild-type timing of pupariation, and a partial suppression of the Hr4Scer\UAS.cQa-mediated lethality. Hr4Scer\UAS.cQa co-expression with Ras85DV12.Scer\UAS represses the hyper-proliferation of the ring gland seen in Ras85D overexpression flies.

      (Ou et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hr4Scer\UAS.cQa
      Hr4UAS.cQa
      Name Synonyms
      Secondary FlyBase IDs
        References (1)