FB2024_03 , released June 25, 2024
Allele: Dmel\midUAS.Tag:FLAG
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General Information
Symbol
Dmel\midUAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0282409
Feature type
allele
Associated gene
Dmel\mid
Associated Insertion(s)
Carried in Construct
P{UAS-mid.flag}
Key Links
Transgenic product class
cDNA
(Formaz-Preston et al., 2012)
wild_type
(Formaz-Preston et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Formaz-Preston et al., 2012)
wild_type
(Formaz-Preston et al., 2012)
Mutagen
in vitro construct
(Formaz-Preston et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-mid.flag}
Cytology
Description

UASt regulatory sequences drive expression of the full-length mid cDNA clone RE27432 which is tagged with Tag:FLAG.

(Formaz-Preston et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
mid
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of midScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 results in 70% embryonic lethality. 46% of the dead embryos have fusions of the midline of the ventral denticle belts.

      Expression of midScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL469B results in just under 50% embryonic lethality. 33% of the dead embryos have fusions of the ventral denticle belts.

      (Formaz-Preston et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of groScer\UAS.cAa does not enhance the penetrance of the embryonic lethality phenotype caused by expression of midScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL469B. However, the fraction of dead embryos which show denticle belt fusions is increased by 33% to 58%.

      (Formaz-Preston et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      midScer\UAS.T:Zzzz\FLAG
      midUAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (3)