FB2024_03 , released June 25, 2024
Allele: Dmel\drdUAS.cSa
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General Information
Symbol
Dmel\drdUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Sansone
FlyBase ID
FBal0282413
Feature type
allele
Associated gene
Dmel\drd
Associated Insertion(s)
Carried in Construct
P{UAS-drd.S}
Also Known As
UAS-drd
Key Links
Transgenic product class
wild_type
(Sansone and Blumenthal, 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Sansone and Blumenthal, 2012)
Mutagen
in vitro construct
(Sansone and Blumenthal, 2012)
Progenitor genotype
Carried in construct
P{UAS-drd.S}
Cytology
Description

UASt regulatory sequences drive expression of the drd coding sequence.

(Sansone and Blumenthal, 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
drd
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4drd-MI15121-TG4.1/drdUAS.cSa rescues drdlwf/drdMI15121-TG4.1

      (Conway et al., 2018)

      drdUAS.cSa/Scer\GAL4DJ626 rescues drdlwf

      (Conway et al., 2018)

      Scer\GAL4Act5C.PI/drdUAS.cSa rescues drdlwf

      (Sansone and Blumenthal, 2012)

      drdUAS.cSa/Scer\GAL4hs.PB rescues drdlwf

      (Sansone and Blumenthal, 2012)
      Partially rescues

      Scer\GAL4DJ717/drdUAS.cSa partially rescues drdlwf

      (Conway et al., 2018)

      drdUAS.cSa/Scer\GAL4hs.PB partially rescues drdlwf

      (Conway et al., 2018)
      Comments

      Expressing drdUAS.cSa under the control of Scer\GAL4drd-MI15121-TG4.1 (the intrinsic Gal4 driver of drdMI15121-TG4.1) rescues adult mortality in drdMI15121-TG4.1/drdlwf flies.

      Expressing drdUAS.cSa under the control of Scer\GAL4DJ626 rescues adult mortality, the presence of holes in the brain, peritrophic membrane formation and defecation rates in drdlwf/drdlwf adult flies.

      Expressing drdUAS.cSa under the control of Scer\GAL4DJ717 rescues the presence of holes in the brain, partially rescues adult mortality and fails to rescue peritrophic membrane formation and defecation rates in drdlwf/drdlwf adult flies.

      Limiting expression of drdUAS.cSa under the control of Scer\GAL4hs.PB to metamorphosis by exposing pupae to the permissive temperature rescues adult mortality, but not peritrophic membrane formation in drdlwf/drdlwf adult flies.

      (Conway et al., 2018)

      The short lived phenotype seen in drdlwf animals is rescued by expression of drdScer\UAS.cSa under the control of Scer\GAL4Act5C.PI.

      Temperature shift experiments using flies carrying drdScer\UAS.cSa and Scer\GAL4hs.PB indicate that expression of drdScer\UAS.cSa during the final 48 hours of metamorphosis completely rescues the short lived phenotype seen in drdlwf animals.

      (Sansone and Blumenthal, 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      drdScer\UAS.cSa
      drdUAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Sansone
      Secondary FlyBase IDs
        References (2)