Expression of Hsap\MFN2R400Q.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel results in a significant reduction in eye area compared to wild type.
Flies expressing Hsap\MFN2R400Q.Scer\UAS under the control of Scer\GAL4tin.CΔ4 have normal climbing activity at 1 week after eclosion, but show reduced climbing activity compared to controls as they age, with the difference first seen approximately 3 weeks after eclosion. At 4 weeks after eclosion, heart tube fractional shortening is significantly depressed in the mutant flies.
Expression of Hsap\MFN2R400Q.Scer\UAS under the control of Scer\GAL4tin.CΔ4 increases mitochondrial heterogeneity and induces mitochondrial fragmentation in adult cardiomyocytes.
Hsap\MFN2R400Q.UAS, Scer\GAL4ey.3.5.Exel is a non-suppressor of visible phenotype of MarfRNAi.cUa.UAS, Scer\GAL4ey.3.5.Exel
Hsap\MFN2R400Q.UAS, Scer\GAL4tin.CΔ4 is a non-suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4
Hsap\MFN2R400Q.UAS, Scer\GAL4tin.CΔ4 is a non-suppressor of adult heart phenotype of MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4
Hsap\MFN2R400Q.UAS, Scer\GAL4tin.CΔ4 is a non-suppressor of mitochondrion | adult stage phenotype of MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4
Hsap\MFN2R400Q.UAS, Scer\GAL4tin.CΔ4 is a non-suppressor of cardial cell | adult stage phenotype of MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4
Co-expression of Hsap\MFN2R400Q.Scer\UAS does not rescue the reduced eye area caused by expression of MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel.
The progressive reduction in climbing ability and depressed heart tube fractional shortening of flies expressing MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4 is not rescued by co-expression of Hsap\MFN2R400Q.Scer\UAS.
Mitochondrial fragmentation in the adult cardiomyocytes caused by expression of MarfmiRNA.cUa.Scer\UAS under the control of Scer\GAL4tin.CΔ4 is not suppressed by co-expression of Hsap\MFN2R400Q.Scer\UAS.