FlyBase Allele Report: Dmel\upd3[Δ]

General Information

Symbol

Dmel\upd3^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0283677

Feature type

allele

Associated gene

Dmel\upd3

Associated Insertion(s)

Carried in Construct

Also Known As

upd3Δ, Δupd3

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Osman et al., 2012)

Mutagen

P-element activity

(Osman et al., 2012)

Progenitor genotype

P{XP}upd3^d00871

(Osman et al., 2012)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 13,464bp deletion that removes the first three exons of upd3.

(Osman et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:18,269,265..18,282,728 [+]

Comment:

13,464 bp deletion resulting from the imprecise excision of P{XP}upd3^d00871. One end of the deletion maps to the insertion site and the other end is upstream.

(Osman et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Delta^UAS.cDa, Not3^KK102144

(Vicente et al., 2018)

modeled by Delta^UAS.cDa, twin^KK108897

(Vicente et al., 2018)

ameliorates hematologic cancer

modeled by hop^Tum

(Bailetti et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult hindgut | conditional

(Cohen et al., 2021)

adult midgut | conditional

(Obata et al., 2018)

embryonic/larval hindgut | wandering third instar larval stage | conditional

(Cohen et al., 2021)

Detailed Description

Statement

Reference

upd3^Δ heterozygosity suppresses the increased pylorus mitotic index in response to injury in the gut at late L3W (by the expression of rpr^UAS.cCb). It also leads to severe shortening, cell loss and/or complete abolishment of the adult hindgut.

(Cohen et al., 2021)

upd3^Δ mutant adults fail to increase the level of cell proliferation (number of EdU+ cells) in the posterior midgut epithelium upon refeeding (starvation followed by 24h of refeeding) as is normally observed in controls.

(Obata et al., 2018)

Unlike wild-type, the survival of adult upd3^Δ mutants is not reduced by lipid-rich diet compared to flies kept on standard diet.

Expression of upd3^Scer\UAS.cUa under the control of Scer\GAL4^Hml.Δ in the upd3^Δ hemizygote background reduces the lifespan of upd3^Δ hemizygote males on a standard diet.

(Woodcock et al., 2015)

upd3^Δ/upd3^Δ adult flies have an increased survival after bacterial inoculation with Providencia rettgeri, but no obvious changes when infected with Enterococcus faecalis.

(Chambers et al., 2014)

Mutants do not show any major developmental defects.

The response of increased proliferation of intestinal stem cells which is seen in wild-type flies after oral infection with Erwinia carotovora carotovora 15 is reduced in mutant flies compared to wild type.

Mutant flies show reduced survival compared to controls after oral infection with Erwinia carotovora carotovora 15.

(Osman et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

upd3^Δ has abnormal immune response | adult stage phenotype, enhanceable by upd2^Δ

(Osman et al., 2012)

Enhancer of

Statement

Reference

upd3^Δ, upd3^Δ, upd2^Δ3-62, upd2^Δ3-62 is an enhancer of visible | adult stage phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

upd3^Δ, upd3^Δ, upd2^Δ3-62, upd2^Δ3-62 is an enhancer of abnormal size | adult stage phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

upd3^Δ, upd3^Δ, upd2^Δ3-62, upd2^Δ3-62 is an enhancer of visible phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

NOT Enhancer of

Statement

Reference

upd2^Δ, upd2[+], upd3^Δ, upd3[+] is a non-enhancer of visible | adult stage phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa

(Worley et al., 2018)

Suppressor of

Statement

Reference

upd3^Δ is a suppressor of abnormal developmental rate phenotype of BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544

(Romão et al., 2021)

upd3^Δ/upd2^Δ is a suppressor | partially of increased occurrence of cell division | recessive | adult stage phenotype of SH3PX1^d1

(Zhang et al., 2019)

upd3^Δ is a suppressor | partially of visible | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, twin^KK108897

(Vicente et al., 2018)

upd3^Δ is a suppressor | partially of neoplasia | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, twin^KK108897

(Vicente et al., 2018)

upd3^Δ/upd2^Δ is a suppressor | partially of visible | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, twin^KK108897

(Vicente et al., 2018)

upd3^Δ/upd2^Δ is a suppressor | partially of neoplasia | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, twin^KK108897

(Vicente et al., 2018)

upd3^Δ is a suppressor of visible | adult stage phenotype of Delta^UAS.cDa, Not3^KK102144, Scer\GAL4^ey.PH

(Vicente et al., 2018)

upd3^Δ is a suppressor of neoplasia | adult stage phenotype of Delta^UAS.cDa, Not3^KK102144, Scer\GAL4^ey.PH

(Vicente et al., 2018)

upd3^Δ, upd2[+], upd3[+], upd2^Δ is a suppressor of visible | adult stage phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

upd3^Δ/upd3[+] is a suppressor | partially of visible | adult stage phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

upd3^Δ is a suppressor | partially of increased occurrence of cell division phenotype of Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^NP3253

(Kenmoku et al., 2016)

upd3^Δ/upd3^Δ is a suppressor | partially of neoplasia phenotype of Sox21a⁶

(Zhai et al., 2015)

NOT Suppressor of

Statement

Reference

upd2^Δ, upd2[+], upd3^Δ, upd3[+] is a non-suppressor of visible | adult stage phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa

(Worley et al., 2018)

Other

Statement

Reference

upd2^Δ, upd3^Δ has abnormal immune response phenotype

(Ahlers et al., 2019)

upd2^Δ, upd3^Δ has increased mortality | conditional phenotype

(Ahlers et al., 2019)

upd2^Δ, upd3^Δ has viable phenotype

(Osman et al., 2012)

upd2^Δ, upd3^Δ has fertile phenotype

(Osman et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

upd3^Δ, upd3^Δ, upd2^Δ3-62, upd2^Δ3-62 is an enhancer of eye phenotype of Bub3^GD9924, Scer\GAL4^ey.PH

(Clemente-Ruiz et al., 2016)

NOT Enhancer of

Statement

Reference

upd2^Δ, upd2[+], upd3^Δ, upd3[+] is a non-enhancer of wing | ectopic phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa

(Worley et al., 2018)

upd3^Δ is a non-enhancer of adult anterior midgut phenotype of Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^NP3253

(Kenmoku et al., 2016)

Suppressor of

Statement

Reference

upd3^Δ/upd2^Δ is a suppressor | partially of adult midgut epithelium phenotype of SH3PX1^d1

(Zhang et al., 2019)

upd3^Δ is a suppressor | partially of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, twin^KK108897

(Vicente et al., 2018)

upd3^Δ/upd2^Δ is a suppressor | partially of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, twin^KK108897

(Vicente et al., 2018)

upd3^Δ is a suppressor of eye phenotype of Delta^UAS.cDa, Not3^KK102144, Scer\GAL4^ey.PH

(Vicente et al., 2018)

upd3^Δ/upd3[+] is a suppressor | partially of wing | ectopic phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

upd3^Δ, upd2[+], upd3[+], upd2^Δ is a suppressor of wing | ectopic phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

upd3^Δ is a suppressor | partially of intestinal stem cell phenotype of Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^NP3253

(Kenmoku et al., 2016)

upd3^Δ/upd3^Δ is a suppressor | partially of adult anterior midgut epithelium phenotype of Sox21a⁶

(Zhai et al., 2015)

NOT Suppressor of

Statement

Reference

upd2^Δ, upd2[+], upd3^Δ, upd3[+] is a non-suppressor of wing | ectopic phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa, upd1^UAS.cUa

(Worley et al., 2018)

upd3^Δ is a non-suppressor of adult anterior midgut phenotype of Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^NP3253

(Kenmoku et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

upd2^Δ upd3^Δ double mutants show increased mortality as a result of WNV-Kun infection when compared to controls.

(Ahlers et al., 2019)

Expression of egr^UAS.cUa under the control of Scer\GAL4^rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in upd3^Δ/+ or upd2^Δ/+ upd3^Δ/+ background does not lead to ectopic wings compared to controls.

(Worley et al., 2018)

Homozygous upd3^Δ reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

(Zhai et al., 2015)

upd2^Δ further reduces the survival of upd3^Δ flies compared to controls after oral infection with Erwinia carotovora carotovora 15.

(Osman et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

55728

w^* upd3^Δ

55729

w^* upd2^Δ upd3^Δ

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

upd3 null

(Woodcock et al., 2015)

upd3^Delta

(Houtz et al., 2019)

upd3^Δ

(Ding et al., 2021, Nagai et al., 2021, Shin et al., 2020, Ahlers et al., 2019, Bailetti et al., 2019, Zhang et al., 2019, Obata et al., 2018, Vicente et al., 2018, Worley et al., 2018, Kenmoku et al., 2017, Terriente-Félix et al., 2017, Guillou et al., 2016, Kenmoku et al., 2016, Zhai et al., 2015, Osman et al., 2012, Osman et al., 2012)

upd3Δ

(Cohen et al., 2021, Cho et al., 2020, Cho et al., 2018, Clemente-Ruiz et al., 2016)

Δupd3

(Romão et al., 2021, Zhang et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (26)

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