Expression of Scop\tra2^Scer\UAS.cMa in the tarsal region of the foreleg imaginal disc, under the control of Scer\GAL4^rn-GAL4-5 in XX pseduomales and in their brother XY males, both mutant for tra2^B, Df(2R)trix and Scer\GAL80^ts.αTub84B results in a number of defects. The foreleg basitarsus contains several transversal rows, the last one forming the sex comb structure in males and in xx pseudomales mutant for tra2^B. This is composed of dark, thick bristles, and is rotated to lie prallele to the proximal-distal leg axis. females lack the sex comb. A significant reduction is seen in the number of bristles forming the male sex comb structure in the foreleg basitarsus of XX pseudomales raised at 25[o]C compared to those raised at 18[o]C. The sex comb size of these latter pseudomales is the same as the sex comb of their XY brothers whether raised at 18 or 25[o]C. Thus, the Scop\tra2 protein supplies tra2 function in Drosophila.

Df(2R)trix, tra2^B/tra2^B XX pseudomales carrying Scop\tra2^Scer\UAS.cMa under the control of the heat-shock inducible Scer\GAL4^hs.PB exhibit signs of feminization.