FB2024_03 , released June 25, 2024
Allele: Scer\GAL4SLC5A11.1.1
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General Information
Symbol
Scer\GAL4SLC5A11.1.1
Species
S. cerevisiae
Name
FlyBase ID
FBal0284258
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{SLC5A11-GAL4.1.1}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Dus et al., 2013)
Progenitor genotype
Carried in construct
P{SLC5A11-GAL4.1.1}
Cytology
Description

SLC5A11 promoter sequences regulate expression of a GAL4 driver.

(Dus et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
SLC5A11
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult ellipsoid body ring neuron R4 | subset

      Comment: 10 to 13 pairs of neurons

      (Dus et al., 2013)
      ellipsoid body | restricted
      (Dus et al., 2013)
      adult gnathal ganglion | restricted
      (Dus et al., 2013)
      antenna
      (Dus et al., 2013)
      enteroendocrine cell | subset of adult anterior midgut epithelium
      (Dus et al., 2013)
      adult ellipsoid body ring neuron R4
      (Park et al., 2016)
      adult brain | restricted
      (Park et al., 2016)
      Additional Information
      Statement
      Reference

      ScerGAL4SLC5A11.1.1 drives expression in a subset of enteroendocrine cells of the anterior midgut that also express pros.

      (Dus et al., 2013)
       
      Marker for
      Reflects expression of
      SLC5A11
      (Dus et al., 2013)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal chemosensory behavior | adult stage | nutrition conditional, with Bhal\NaChBacUAS.cNa

      (Park et al., 2016)

      abnormal chemosensory behavior | adult stage | nutrition conditional, with Hsap\KCNJ2UAS.cUa.EGFP

      (Park et al., 2016)

      abnormal feeding behavior | adult stage, with Bhal\NaChBacUAS.cNa

      (Park et al., 2016)

      abnormal feeding behavior | adult stage, with Hsap\KCNJ2UAS.cUa.EGFP

      (Park et al., 2016)

      abnormal feeding behavior | adult stage, with SLC5A11GD394

      (Dus et al., 2013)

      abnormal feeding behavior | adult stage, with SLC5A11GD17030

      (Dus et al., 2013)

      abnormal feeding behavior | adult stage |  :nutrition conditional | heat sensitive, with shits.UAS.cUa

      (Park et al., 2016)

      abnormal feeding behavior | adult stage |  :nutrition conditional | heat sensitive, with TrpA1UAS.(B).cKa

      (Park et al., 2016)

      abnormal feeding behavior | adult stage | heat sensitive, with Hsap\KCNJ2UAS.EGFP

      (Dus et al., 2013)

      abnormal feeding behavior | adult stage | heat sensitive, with TrpA1UAS.(B).cKa

      (Park et al., 2016)

      abnormal feeding behavior | adult stage | nutrition conditional, with Bhal\NaChBacUAS.cNa

      (Park et al., 2016)

      abnormal feeding behavior | adult stage | nutrition conditional, with KCNQJF02562

      (Park et al., 2016)

      abnormal feeding behavior | adult stage | nutrition conditional, with SLC5A11I94A.S380A.UAS.cPa

      (Park et al., 2016)

      abnormal feeding behavior | adult stage | nutrition conditional, with SLC5A11UAS.cPa

      (Park et al., 2016)

      abnormal neurophysiology | adult stage, with SLC5A11I94A.S380A.UAS.cPa

      (Park et al., 2016)

      abnormal neurophysiology | adult stage, with SLC5A11UAS.cPa

      (Park et al., 2016)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Activation (using Bhal\NaChBacUAS.cNa) of Scer\GAL4SLC5A11.1.1 cells in adults abolishes the significant decrease in preference for nutritive sugars and the significant decrease in preference for food (vinegar) odor seen in fed versus starved flies, compared to controls; this activation also significantly increases food intake of fed or starved flies, compared to controls.

      Activation (using TrpA1UAS.(B).cKa at 32[o]C) of Scer\GAL4SLC5A11.1.1 cells in adults abolishes the significant decrease in preference for nutritive sugars seen in fed versus starved flies and significantly increases proboscis extension rate in the absence of food, compared to controls.

      Inhibition (using shits.UAS.cUa at 32[o]C) of Scer\GAL4SLC5A11.1.1 cells in adults abolishes the significant increase in preference for nutritive sugars seen in starved versus fed flies, compared to controls.

      Inhibition (using Hsap\KCNJ2UAS.cUa.EGFP) of Scer\GAL4SLC5A11.1.1 cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, significantly reduces food intake of starved adults, compared to controls; this inhibition also abolishes the significant increase in preference for food (vinegar) odor seen in starved versus fed flies, compared to controls.

      (Park et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      KCNQJF02562, Scer\GAL4SLC5A11.1.1 has abnormal feeding behavior | adult stage | nutrition conditional phenotype, non-enhanceable by SLC5A11EY21708

      (Park et al., 2016)
      NOT suppressed by
      Statement
      Reference

      KCNQJF02562, Scer\GAL4SLC5A11.1.1 has abnormal feeding behavior | adult stage | nutrition conditional phenotype, non-suppressible by SLC5A11EY21708

      (Park et al., 2016)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4SLC5A11.1.1
      Name Synonyms
      Secondary FlyBase IDs
        References (3)