UAS regulatory sequences drive expression of fu which is tagged at the N-terminal end with Tag:Palm(hGAP43) followed by CFP.
Expression of fuScer\UAS.T:Hsap\Myr2,T:Avic\GFP-CFP under the control of Scer\GAL4Bx-MS1096 results in wings with the anterior compartment containing ectopic vein tissue associated with supernumerary campaniform sensillae. The flies are unable to emerge from the pupal case.
Scer\GAL4Bx-MS1096, fuUAS.Tag:Palm(hGAP43),CFP has visible phenotype, suppressible by smo[+]/smoD16
Scer\GAL4Bx-MS1096, fuUAS.Tag:Palm(hGAP43),CFP has visible phenotype, non-suppressible by ptcUAS.cJa/Scer\GAL4Bx-MS1096
Scer\GAL4Bx-MS1096, fuUAS.Tag:Palm(hGAP43),CFP has wing phenotype, suppressible by smo[+]/smoD16
fuUAS.Tag:Palm(hGAP43),CFP has wing phenotype, non-suppressible by ptcUAS.cJa/Scer\GAL4Bx-MS1096
smoD16/+ restores an almost wild-type wing phenotype in animals expressing fuScer\UAS.T:Hsap\Myr2,T:Avic\GFP-CFP under the control of Scer\GAL4Bx-MS1096.
Co-expression of ptcScer\UAS.cJa does not suppress the wing defects caused by expression of fuScer\UAS.T:Hsap\Myr2,T:Avic\GFP-CFP under the control of Scer\GAL4Bx-MS1096.
fuUAS.Tag:Palm(hGAP43),CFP/Scer\GAL4Bx-MS1096 partially rescues fu54
Scer\GAL471B/fuUAS.Tag:Palm(hGAP43),CFP partially rescues fu54
Expression of fuScer\UAS.T:Hsap\Myr2,T:Avic\GFP-CFP under the control of either Scer\GAL4Bx-MS1096 or Scer\GAL471B rescues the reduction in the L3-L4 intervein region that is seen in fu54 flies, although the rescued wings show some wing vein abnormalities.