FB2024_03 , released June 25, 2024
Allele: Dmel\p53UAS.DΔNp53
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General Information
Symbol
Dmel\p53UAS.DΔNp53
Species
D. melanogaster
Name
FlyBase ID
FBal0285118
Feature type
allele
Associated gene
Dmel\p53
Associated Insertion(s)
Carried in Construct
PBac{UAS-p53.ΔNp53}
Also Known As
UAS-DΔNp53
Key Links
Transgenic product class
wild_type
(Dichtel-Danjoy et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Dichtel-Danjoy et al., 2013)
Mutagen
in vitro construct
(Dichtel-Danjoy et al., 2013)
Progenitor genotype
Carried in construct
PBac{UAS-p53.ΔNp53}
Cytology
Description

UASt regulatory sequences drive expression of the DΔNp53 isoform of p53.

(Dichtel-Danjoy et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
p53
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing p53UAS.DΔNp53 under the control of Scer\GAL4ninaE.PT induces apoptosis (cleaved Dcp1) and loss of photoreceptor cells in the adult retina.

      The expression of p53UAS.DΔNp53 under the control of Scer\GAL4ninaE.PT induces the formation of autophagosomes (Atg8a puncta and TEM) in the photoreceptor cells of the retina, as compared to controls. There are apparent defects in autophagic flux ( autophagosome:autolysosome ratio, inferred from the yellow:red ratio of mCherry-GFP-Atg8a puncta).

      (Robin et al., 2019)

      Expression of p53UAS.DΔNp53 under the control of Scer\GAL4sd.PU causes an increase in apoptosis (activated caspase-3 staining), a significant increase in proliferation (PH3 staining), protruding overgrowths and disruption of the dorso-ventral boundary in the wing pouch domain, compared to controls, with no proliferation change in the notum region; the entire wing disc is reduced in size compared to controls; adult wings of this genotype have clear morphological defects, including extreme notching when raised at 17 celsius.

      (Simón et al., 2014)

      Expression of p53Scer\UAS.DΔNp53 under the control of Scer\GAL4Bx-MS1096 induces apoptosis in wing discs. Increased cell proliferation and tissue folding is seen compared to controls. An increase in apoptosis is also seen when p53Scer\UAS.Dp53 is expressed in a p535A-1-4 mutant background.

      (Dichtel-Danjoy et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Scer\GAL4sd.PU, p53UAS.DΔNp53 has wing disc phenotype, enhanceable by N[+]/N55e11

      (Simón et al., 2014)

      Scer\GAL4sd.PU, p53UAS.DΔNp53 has wing pouch phenotype, enhanceable by Dp(1;3)DC109/+

      (Simón et al., 2014)
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Addition of N55e11/+ to individuals expressing p53UAS.DΔNp53 under the control of Scer\GAL4sd.PU exacerbates the decrease in wing disc size, reduces wing pouch protruding overgrowths and results in much smaller adult wings compared to controls; addition of Dp(1;3)DC109/+ instead prevents the decrease in wing disc size, exacerbates wing pouch protruding overgrowths and results in adult wings that have blisters and overgrowths that are not seen in controls.

      Additional expression of NTRiP.cUa in individuals expressing p53UAS.DΔNp53 under the control of Scer\GAL4sd.PU reduces wing pouch protruding overgrowths and suppresses the size decrease of the wing disc.

      Additional expression of Diap1UAS.cUa in individuals expressing p53UAS.DΔNp53 under the control of Scer\GAL4sd.PU reduces cell death (activated caspase 3 staining), but does not prevent overgrowths in the wing pouch; these Diap1UAS.cUa and p53UAS.DΔNp53 co-expressing wing discs become smaller upon additional expression of NTRiP.cUa.

      (Simón et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference

      Coexpression of BacA\p35UAS.cUa and p53UAS.DΔNp53 under the control of Scer\GAL4nub.PU results in tissue overgrowths in the wing disc; this phenotype is reduced by the additional expression of NTRiP.cUa.

      (Simón et al., 2014)
      Complementation and Rescue Data
      Partially rescued by

      p53UAS.DΔNp53 is partially rescued by p53TRiP.cUa/Scer\GAL4sd.PU

      (Simón et al., 2014)
      Partially rescues

      p53UAS.DΔNp53/Scer\GAL4nanos.PU partially rescues p53E8

      (Napoletano et al., 2017)
      Comments

      The expression of p53Scer\UAS.DΔNp53 under the control of Scer\GAL4nos.PU suppresses the significant proportion of hyperplastic adult testes in p53E8 homozygotes, as well as the increased necrosis at their apical tip.

      (Napoletano et al., 2017)

      Additional expression of p53TRiP.cUa mostly rescues the adult wing morphological defects caused by expression of p53UAS.DΔNp53 under the control of Scer\GAL4sd.PU.

      (Simón et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      p53Scer\UAS.DΔNp53
      p53UAS.DΔNp53
      Name Synonyms
      Secondary FlyBase IDs
        References (5)